TUNEL Label Mix
ROCHE/11767291910 - sufficient for 30 tests, pkg of 3 × 550 μL
Synonym: transferase dUTP nick end labeling; tunel
Product Type: Chemical
| color | colorless |
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 3 × 550 μL |
| Quality Level | 100  |
| solubility | water: miscible |
| storage temp. | −20°C |
| usage | sufficient for 30 tests |
| Application: | The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL Enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues. |
| Application: | TUNEL Label Mix has been used for the determination of apoptosis using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay and DNA nick-end labeling by the TUNEL method. |
| General description: | The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working solution: TUNEL Label is used in combination with the TUNEL Enzyme to prepare the TUNEL reaction mixture. For one test: Mix 45 μl TUNEL Label with 5 μl TUNEL Enzyme prior to use. For negative control use 50 μl/test TUNEL Label only. Storage conditions (working solution): Note: The TUNEL reaction mixture (45 μl TUNEL Label with 5 μl TUNEL Enzyme for 1 test) should be prepared just before use, and should not be stored. Keep the TUNEL reaction mixture on ice until use. |
| Symbol |   GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H350i - H411 |
| Precautionary statements | P201 - P273 - P280 - P308 + P313 - P391 - P501 |
| RIDADR | UN 1556 6.1 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41105500 |