TUNEL Enzyme
ROCHE/11767305001 - from calf thymus recombinant in E. coli
Synonym: transferase dUTP nick end labeling; tunel
Product Type: Chemical
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 2 × 50 μL |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| usage | sufficient for 20 tests |
| Application: | The enzyme solution contains terminal deoxynucleotidyl transferase from calf thymus, recombinant in E. coli, needed to perform the TUNEL reaction for detecting apoptosis in situ. The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues. |
| General description: | TUNEL enzyme solution, containing terminal deoxynucleotidyl transferase from calf thymus recombinant in E. coli. One of the two components required for performing the TUNEL reaction to detect apoptosis in situ. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Terminal deoxynucleotidyl transferase (TdT) in an optimized concentration, dissolved in reaction buffer. Ready-to-use with TUNEL Label. |
| Preparation Note: | Working solution: TUNEL Enzyme is used in combination with TUNEL Label to prepare the TUNEL reaction mixture. To produce a TUNEL solution sufficient for one test: • Mix 5μl TUNEL Enzyme with 45μl TUNEL Label prior to use. • For negative control use 50μl/test TUNEL Label only. Storage conditions (working solution): Note: The TUNEL reaction mixture (45μl TUNEL Label with 5μl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41105600 |