DIG Easy Hyb™ Granules
ROCHE/11796895001 - powder, suitable for hybridization
Synonym: DIG system
Product Type: Chemical
form | powder |
greener alternative category | Aligned |
greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
manufacturer/tradename | Roche |
Quality Level | 100 |
storage temp. | 20-25°C |
technique(s) | hybridization: suitable |
usage | sufficient for 6 x 100 mL |
Application: | DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits. |
General description: | DIG Easy Hyb is a nontoxic, ready-to-use hybridization buffer. DIG Easy Hyb does not contain formamide, but facilitates lowering of hybridization temperature comparable to buffers that contain 50% formamide. |
General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
Legal Information: | DIG Easy Hyb is a trademark of Roche |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Preparation Note: | Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required. Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions. |
Preparation Note: | Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use. |
Symbol | GHS05 |
Signal word | Danger |
Hazard statements | H315 - H318 |
Precautionary statements | P264 - P280 - P302 + P352 - P305 + P351 + P338 + P310 - P332 + P313 - P362 + P364 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Storage Temp. | 20-25°C |
UNSPSC | 41105326 |