DIG Easy Hyb™ Granules
ROCHE/11796895001 - powder, suitable for hybridization
Synonym: DIG system
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| agency | suitable for |
| color | white |
| form | powder |
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| manufacturer/tradename | Roche |
| pH | 7,0 |
| Quality Level | 100 |
| solubility | water: soluble |
| storage condition | (Keep container tightly closed in a dry and well-ventilated place.) |
| storage temp. | 20-25°C |
| suitability | suitable for nucleic acid hybridization |
| sustainability | Greener Alternative Product |
| technique(s) | hybridization: suitable |
| usage | sufficient for 6 x 100 mL |
| Application: | DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits. |
| Features and Benefits: | • DIG Easy Hyb™ is a convenient ready-to-use hybridization buffer, saving time and effort. • DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers. |
| General description: | DIG Easy Hyb™ is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
| Legal Information: | DIG Easy Hyb is a trademark of Roche |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required. Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions. |
| Preparation Note: | Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H315 - H318 |
| Precautionary statements | P264 - P280 - P302 + P352 - P305 + P351 + P338 + P310 - P332 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 20-25°C |
| UNSPSC | 41105326 |