mini Quick Spin DNA Columns
ROCHE/11814419001 - pkg of 50 columns
Synonym: DNA column
Product Type: Chemical
manufacturer/tradename | Roche |
packaging | pkg of 50 columns |
Quality Level | 100 |
storage temp. | 2-8°C |
Application: | Ready-to-use, disposable, microfuge-compatible columns designed for: • Removal of unincorporated radiolabeled nucleotides from DNA labeled by nick translation, end-labeling, polymerization reactions, or other labeling techniques • Removal of excess dye-labeled dideoxynucleotide terminators prior to fluorescent sequencing The mini Quick Spin columns are designed for use with variable-speed, fixed-angle microcentrifuges. |
Features and Benefits: | Ready-to-use disposable columns designed to quickly and efficiently remove unincorporated nucleotides from freshly prepared radiolabeled or fluorescently labeled DNA (=20 bp). mini Quick Spin Columns can be used in any standard microcentrifuge. • Save time and effort with ready-to-use columns. • Purify more nucleic acid in less time - process up to 75 μl of sample in only 7 minutes. • Minimize sample loss while maximizing yield - short and effective two-step procedure. • Easy-to-use - column and cap allow for rapid resuspension of the gel matrix, and the bottom tip can be quickly snapped off. • Ensure optimal performance and reproducibility with quality tested columns: High recovery of radiolabeled DNA, Maximum retention of unincorporated nucleotides & Absence of DNases |
General description: | Contents: Pre-packed, pre-swollen, microfuge-compatible columns with a cap and snap-off bottom tip. Suspension of Sephadex G-50 in STE buffer (10 mM Tris-HCI, pH 7.5, 1 mM EDTA, 100 mM NaCl). |
General description: | Principle: The mini Quick Spin gel filtration columns separate molecules based on their relative size. This rapid chromatographic separation may be done in a conventional tabletop microcentrifuge. During centrifugation, mini Quick Spin columns allow larger molecules (DNA, RNA, or oligonucleotides) to pass through quickly while retaining smaller molecules, such as unincorporated nucleotides. Recovery of labeled DNA: =90% Retention of unincorporated nucleotides: >99% Sample size: 20 – 75 μl Time required: 7 minutes Reactions/standard sample: 50 |
General description: | Ready-to-use, microcentrifuge-compatibl |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Quality: | DNases are not detected, according to the current Quality Control procedures. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Storage Temp. | 2-8°C |
UNSPSC | 41115700 |