Anti-HA Affinity Matrix
ROCHE/11815016001 - from rat IgG1
Synonym: affinity matrix, anti-ha
Product Type: Chemical
Anti-HA Affinity Matrix
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| capacity | 2-8 nmol/mL binding capacity |
| clone | 3F10, monoclonal |
| conjugate | agarose conjugate |
| epitope sequence | YPYDVPDYA |
| form | slurry |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 1 mL (settled resin volume) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Analysis Note: | Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody. |
| Analysis Note: | Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase. |
| Application: | Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by: • Western blotting using the Anti-HA antibody • Silver staining (or similar protein stain) • In coimmunoprecipitation. |
| Application: | Use Anti-HA Affinity Matrix for: • Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extracts • Affinity column purification of HA-tagged proteins from crude protein extracts |
| Biochem/physiol Actions: | Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal). |
| Features and Benefits: | • Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity. • Use nondenaturing conditions for elution of your HA-tagged protein of choice. • Purify even rarely expressed HA-tagged proteins. • Highly specific to YPYDVPDYA. • Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion. • Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems. |
| General description: | Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal) |
| Immunogen: | Amino acids 98-106 from the human influenza virus hemagglutinin protein |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | 1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |