Pefabloc® SC PLUS
ROCHE/11873628001 - solution, suitable for electrophoresis, solubility: 0.1 M in water, soluble, pkg of 2 × 25 mL (1 g)
Synonym: PSC-protector; serine protease inhibitor
Product Type: Chemical
Mass spectrograms showing the covalent interaction between insulin and the protease inhibitor Pefabloc® SC. Diagram A is the insulin blank. At 1 mM Pefabloc SC, the formation of the binding is visible as a second peak formation (Diagram B) Higher concentrations of the protease inhibitor result in more than one interaction per insulin molecule (Diagram C). The PSC-Protector solution eliminates this covalent interaction, even at the highest concentrations (Diagram D). Matrix peaks are subtracted.
Chymotrypsin inhibition with TM="Pefabloc"] SC in the absence (A) and presence (B) of the PSC-protector. Results show no change in the effectiveness of inhibition.
| concentration | 0.1-1.0 mg/mL |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | 239.5 |
| mp | 175-185 °C |
| packaging | pkg of 2 × 25 mL (1 g) |
| solubility | water: soluble 0.1 M |
| storage temp. | 2-8°C |
| technique(s) | electrophoresis: suitable |
| Features and Benefits: | • Take advantage of a simplified two-reagent system. Use the PSC-Protector solution to prevent covalent binding between proteins and Pefabloc® SC, even at high concentrations, extended incubation times, and at alkaline pH (see Figure 1 and Figure 2). • Benefit from an easy-to-use inhibitor. Add water-soluble Pefabloc® SC directly to aqueous buffers. • Avoid hazardous compounds. Obtain non-toxic protease inhibition without risk to you, or those around you. • Ensure protection with improved stability. Achieve consistent protease inhibition even at pH levels above 7.0 and temperatures above +4°C. • Maximize inhibition. Be certain that your levels of active inhibitor are high enough by using a product that is reliably soluble and stable. |
| General description: | Set consists of Pefabloc® SC and the corresponding amount of PLUS additive to avoid covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH. Choose Pefabloc® SC for all applications where the general inhibition of serine proteases is desired. With its high stability and irreversible inhibition mechanism, Pefabloc® SC protects proteins throughout procedures such as: • Extraction processes (from animal tissues or cells, plants, bacteria, yeast, and fungi) • Subsequent purification steps • Varied sample storage conditions • Downstream protein detection (e.g., western blotting, reporter gene analysis, etc.) • Biochemical studies where proteins are required. Use Pefabloc® SC to inactivate proteinase K, for example, during pulsed-field gel electrophoresis (PFGE). With this technique, isolating the genomic DNA requires proteinase K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc® SC inhibits proteinase K, and protects the stability of restriction enzymes used for further DNA analysis. |
| General description: | The Pefabloc® SC PLUS set combines the serine protease inhibitor Pefabloc® SC with a uniquely formulated Pefabloc® SC protector (PSC-Protector), adding additional convenience and reliability to the safe, stable protease inhibition provided by Pefabloc® SC . Recent findings indicate that sulfonyl-type serine protease inhibitors such as Pefabloc® SC and PMSF can bind covalently to proteins. This can occur when the inhibitors are used in high concentrations, or during extended incubation times under alkaline conditions. This interaction adversely affects the tyrosine and lysine residues of a protein, as well as the free amino terminus. The PLUS additive (PSC-Protector) included with this set prevents covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH. |
| Legal Information: | Pefabloc is a registered trademark of Pentapharm |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | Set consists of Pefabloc® SC and the corresponding amount of PLUS additive to avoid covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH. Choose Pefabloc® SC for all applications where the general inhibition of serine proteases is desired. With its high stability and irreversible inhibition mechanism, Pefabloc® SC protects proteins throughout procedures such as: • Extraction processes (from animal tissues or cells, plants, bacteria, yeast, and fungi) • Subsequent purification steps • Varied sample storage conditions • Downstream protein detection (e.g., western blotting, reporter gene analysis, etc.) • Biochemical studies where proteins are required. Use Pefabloc® SC to inactivate proteinase K, for example, during pulsed-field gel electrophoresis (PFGE). With this technique, isolating the genomic DNA requires proteinase K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc® SC inhibits proteinase K, and protects the stability of restriction enzymes used for further DNA analysis. |
| Packaging: | Set containing two components |
| Specificity: | Specific, potent, and irreversible inhibitor of serine proteases. The inhibitory activity of Pefabloc® SC is comparable to PMSF or DFP; however, Pefabloc® SC is non-toxic. Specifically inhibits serine proteases and prevents nonspecific covalent modification of proteins as tested by mass spectrometry. |
| Symbol |   GHS02,GHS05 |
| Signal word | Danger |
| Hazard statements | H226 - H314 |
| Precautionary statements | P210 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P370 + P378 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 2 |
| mp | 175-185 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |
| Components | Pefabloc SC, 1 g; PSC-Protector solution, 2 x 25 mL ready-to-use |