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Anti-His6-Peroxidase

ROCHE/11965085001 - from mouse IgG1

Synonym: antibody

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-11965085001 50 units
 $715.00
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antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone BMG-his-1, monoclonal
conjugate peroxidase conjugate
form lyophilized
isotype IgG1
manufacturer/tradename Roche
packaging pkg of 50 U
Quality Level 100 
storage temp. 2-8°C
Analysis Note: Function test: Western blot using extracts from cell line expressing a recombinant His6-tagged protein.
Application: Anti-His6-Peroxidase has been used in in vitro serum stability of fusion toxins, pull-down assay and western blotting.
Application: Anti-His6-Peroxidase is used for the detection of His6-tagged proteins in:
• ELISA (enzyme-linked immunosorbent assay)
• Western blot
Biochem/physiol Actions: Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the epitopesequence. However, Anti-His6-Peroxidase preferentially recognizes the C-terminal His6 epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
Biochem/physiol Actions: The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.
General description: Anti-His6-Peroxidase is a monoclonal antibody to His6-tagged proteins, conjugated to horseradish peroxidase. Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the epitope-sequence location; however, it preferentially recognizes the C-terminal His6 epitope with high sensitivity.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Working concentration: Working concentration of conjugate depends on application and substrate.


The following concentrations should be taken as a guideline:
• ELISA: 100 mU/ml
• Western blot: 100mU/ml
Preparation Note: Add 1 ml double-distilled water to a final concentration of 50 U/ml.
Reconstitution should be performed for at least 10 minutes.
Storage Temp. 2-8°C
UNSPSC 12352203

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