Anti-His6-Peroxidase
ROCHE/11965085001 - from mouse IgG1
Synonym: antibody
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BMG-his-1, monoclonal |
| conjugate | peroxidase conjugate |
| form | lyophilized |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 U |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Anti-His6-Peroxidase has been used in in vitro serum stability of fusion toxins, pull-down assay and western blotting. |
| Application: | Anti-His6-Peroxidase is used for the detection of His6-tagged proteins in: • ELISA (enzyme-linked immunosorbent assay) • Western blot |
| General description: | Anti-His6-Peroxidase is a monoclonal antibody to His6-tagged proteins, conjugated to horseradish peroxidase. Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the epitope-sequence location; however, it preferentially recognizes the C-terminal His6 epitope with high sensitivity. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • ELISA: 100 mU/ml • Western blot: 100mU/ml |
| Quality: | Function test: Western blot using extracts from cell line expressing a recombinant His6-tagged protein. |
| Reconstitution: | Add 1 ml double-distilled water to a final concentration of 50 U/ml. Reconstitution should be performed for at least 10 minutes. |
| Specificity: | Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the epitopesequence. However, Anti-His6-Peroxidase preferentially recognizes the C-terminal His6 epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used. |
| Specificity: | The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H412 - H317 |
| Precautionary statements | P273 - P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |