Anti-HA-Peroxidase, High Affinity
ROCHE/12013819001 - from rat IgG1
Synonym: antibody
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | clone 3F10, monoclonal |
| conjugate | peroxidase conjugate |
| epitope sequence | YPYDVPDYA |
| form | lyophilized (clear, colorless solution after reconstitution) |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 25 U (25 μg) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | • Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA • Western blot Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling. |
| Biochem/physiol Actions: | Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal). |
| General description: | Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase. |
| General description: | The Anti-HA High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5, which was originally used to study how the immune system recognizes the influenza hemagglutinin protein, a surface glycoprotein required for infectivity of the human virus. However, the principal use of the Anti-HA antibody is the detection and purification of proteins whose encoding DNA sequences have been fused to the HA epitope sequence by recombinant techniques, that is epitope tagging. The ability to prepare such epitope-tagged proteins and locate them with the Anti-HA antibody in subsequent experiments has enabled researchers to determine: • The size, cellular localization, and abundance of proteins produced by newly discovered genes. • Post-translational modifications of proteins. • The movement of proteins within cell membranes. • The identity of proteins within functional protein complexes. • The function of proteins that are unstable, difficult to purify, or share epitopes with a number of other proteins. |
| Immunogen: | The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Stabilizers: proteinaceous stabilizers Working concentration: The working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • Dot blot: 50 mU/ml • ELISA: 25 mU/ml • Western blot: 50 mU/ml |
| Preparation Note: | Add 1.0 ml double-distilled water for a final concentration of 25 U/mL. Rehydrate for 10 min prior to use. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |