Anti-HA-Peroxidase, High Affinity
ROCHE/12013819001 - from rat IgG1
Synonym: antibody
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | clone 3F10, monoclonal |
| conjugate | peroxidase conjugate |
| epitope sequence | YPYDVPDYA |
| form | lyophilized (clear, colorless solution after reconstitution) |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 25 U (25 μg) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | • Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA • Western blot Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling. |
| Biochem/physiol Actions: | Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal). |
| General description: | Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase. |
| Immunogen: | The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Stabilizers: proteinaceous stabilizers Working concentration: The working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • Dot blot: 50 mU/ml • ELISA: 25 mU/ml • Western blot: 50 mU/ml |
| Preparation Note: | Add 1.0 ml double-distilled water for a final concentration of 25 U/mL. Rehydrate for 10 min prior to use. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |