DIG Northern Starter Kit
ROCHE/12039672910 - suitable for Northern blotting, sufficient for 10 labeling reactions
Synonym: DIG system; Northern blot
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| manufacturer/tradename | Roche |
| parameter | 68 °C optimum reaction temp. |
| Quality Level | 100 |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| technique(s) | Northern blotting: suitable |
| usage | sufficient for 10 blots (blots of 10 x 10 cm2) |
| sufficient for 10 labeling reactions |
| Application: | DIG northern starter kit has been used in northern blot hybridization to analyse hepatitis B virus (HBV) RNAs, citrus leprosis virus cytoplasmic type 2 (CiLV-C2) RNAs. |
| Application: | The DIG Northern Starter Kit produces DIG-labeled RNA probes that can be used in conjunction with the supplied chemiluminescent detection reagents for northern blotting techniques. Using linearized DNA as a template, SP6, T3, or T7 RNA Polymerases are used to incorporate DIG-11-UTP into the RNA transcript. After labeling, the DIG-labeled probe is immediately ready for use in hybridization. For convenience, DIG Easy Hyb can be used for hybridization. The DIG Easy Hyb granules are easily reconstituted by adding 64 ml DEPC-treated (RNase-free) water directly to the bottle (once reconstituted, DIG Easy Hyb is stable for 1 month at room temperature). After hybridization, the hybridization solution containing the DIG-labeled RNA probe can be stored at -15 to -25 °C for future re-use (up to 1 year). |
| General description: | The DIG Northern Starter Kit is designed for the novice DIG system user. It contains the reagents proven to provide successful, reproducible results in northern blots. Additionally, the more convenient forms of standard DIG system products are included (e.g., CDP Star, ready-to-use). The small number of reactions allows the user to gain a firm foundation using the DIG system, then "graduate" to the standard pack sizes. For optimum success, use this kit with the DIG Wash and Block Buffer Set.This kit is used for the generation of single-stranded, DIG-labeled RNA probes and chemiluminescent detection. Labeled probes are synthesized by the in vitro transcription method. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 11 components. 1 kit for up to 10 labeling reactions and detection of 10 blots, blots of 10×10cm2, reactions of μg DNA, yielding approx. 20μg labeled RNA, each |
| Packaging: | Sensitivity and specificity: Using 1μg of linearized template DNA, the labeling reaction typically yields 20μg of newly synthesized DIG-labeled RNA within 1 hour. The DIG-labeled RNA probe can detect 0.1pg of homologous DNA or RNA in a dot blot. Rare mRNAs can be detected in 0.1μg of total RNA. |
| Preparation Note: | Working solution: Note: Use sterile, RNase-free solutions and equipment Assay Time: 9 hours 30 minutes Sample Materials: • Linearized plasmid, including the appropriate RNA polymerase promoter sequence (SP6, T3, T7). • Specially prepared PCR productWorking solution: Note: Use sterile, RNase-free solutions and equipment. Note: The length of the region to be transcribed should be in the range of 200 to 1,000 bp. To avoid RNase contamination the DNA must be phenolized. |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 |
| Precautionary statements | P264 - P270 - P280 - P301 + P312 + P330 - P305 + P351 + P338 + P310 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41105500 |
| Components | Labeling Mix 5x concentrated; Transcription Buffer 5x concentrated; SP6 RNA Polymerase 20 U/μl; T7 RNA Polymerase 20 U/μl; T3 RNA Polymerase 20 U/μl; Anti-Digoxigenin-AP antibody, Fab fragments 750 U/ml; DNase I, RNase free 10 U/μl; CDP-Star ready-to-use; Actin RNA Probe, DIG-labeled Antisense Probe, length 588 bases 10 ng/μl; DIG Easy Hyb Granules; Blocking Solution 10x concentrated |