CDP-Star®, ready-to-use
ROCHE/12041677001 - >98%, solution, suitable for dot blot, suitable for Northern blotting, suitable for Southern blotting
Product Type: Chemical
CDP-Star, ready-to-use
| assay | >98% |
| description | Disodium 2-chloro-5-(4-methoxyspir |
| form | solution |
| manufacturer/tradename | Roche |
| packaging | bottle of 2 × 50 mL ([0.25 mM]) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | dot blot: suitable |
| Northern blotting: suitable | |
| Southern blotting: suitable |
| Application: | Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots: • Southern blot • Northern blot • Dot blot • Colony and plaque hybridization • Gel shift assays |
| Application: | Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots: • Southern blot • Northern blot • Dot blot • Colony and plaque hybridization • Gel shift assays • Chemiluminescent detection. |
| Features and Benefits: | Contents 0.25 mM solution (0.124 mg/ml), ready-to-use, colorless solution |
| Features and Benefits: | CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems. • Save time with a fast, ultra-sensitive substrate. • Easily strip and reprobe membranes. • Take multiple exposures of up to two days for each experiment. Reuse the substrate by filtering and storing in sodium azide. |
| Legal Information: | CDP-Star is a registered trademark of Tropix, Inc. |
| Other Notes: | • First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result. • No preincubation step required. • Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background). • Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film. • Exposure times are shorter than with CSPD. • Do not use nitrocellulose membranes. Repeat exposures can be made up to two days after the addition of substrate. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable Maleic Acid Buffer: 15 to 25 °C, stable Detection Buffer: 15 to 25 °C, stable Blocking Solution: always prepare freshly Antibody Solution: 2 to 8 °C |
| Publishing: | Purity: Purified by HPLC. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | >98% |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |