High Fidelity PCR Master
ROCHE/12140314001 - sufficient for ≤200 reactions, kit of 1 (2 components), suitable for PCR
Synonym: High fidelity PCR | PCR
Product Type: Chemical
Typical results. Lanes 1: MWM III 2: High Fidelity PCR Master 1.1 kb collagen fragment 3: High Fidelity PCR Master 1.7 kb tPA fragment 4: High Fidelity PCR Master 2.9 kb P53 fragment 5: High Fidelity PCR Master 4.8 kb tPA fragment 6: MWM III
Advantages of High Fidelity PCR Master
| feature | dNTPs included |
| High Fidelity PCR | |
| hotstart: no | |
| input | purified DNA |
| manufacturer/tradename | Roche |
| packaging | kit of 1 (2 components) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | PCR: suitable |
| usage | sufficient for ≤200 reactions |
| Application: | • Amplify longer fragments with more fidelity up to 5 kb. • Reduce setup time. Store at +2 to +8ºC – ready for immediate use – without thawing. • Minimize contamination with two pipetting steps: Add primers and template. |
| Application: | Choose the convenient High Fidelity PCR Master, a double-concentrated, ready-to-use mix, which combines Expand High Fidelity PCR System with PCR-Grade dNTPs, MgCl2, and an optimized reaction buffer. The High Fidelity PCR Master delivers twice the yield and three times better fidelity than Taq DNA Polymerase alone.High yield and increased fidelity compared to Taq DNA Polymerase. Use the High Fidelity PCR Master for: • PCR up to 5 kb • RT-PCR up to 5 kb High Fidelity PCR Master is stable at +2 to +8°C, allowing PCR without time-consuming reagent thawing. |
| General description: | The 5′→3′ polymerase activity of Taq DNA Polymerase and the 3′→ 5′ exonuclease of the proofreading polymerase combine for improved performance and results; 3′→5′ exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase. PCR products have both blunt ends and 3′ single A-overhangs just like using Taq DNA polymerase, ideal for blunt end cloning or T/A cloning. The High Fidelity PCR Master is a ready-to-use, double-concentrated master mix that contains all the reagents required to perform PCR (except template and primer). Water, PCR Grade, is also supplied. This powerful specific enzyme blend of the High Fidelity PCR Master is designed to give PCR products with high yield, high fidelity, and high specificity from episomal and genomic DNA. The special enzyme blend is composed of two thermostable polymerases: Taq DNA Polymerase and a thermostable polymerase with proofreading activity. Due to the inherent 3′-5′ exonuclease activity of the proofreading polymerase, the High Fidelity PCR Master results in approximately three times the fidelity and approximately twice the yield of Taq DNA Polymerase alone. |
| Legal Information: | Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 2 components. |
| Quality: | Each lot is PCR function tested using human genomic DNA and primers specific for a 4.8 kb fragment of the tPA gene. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106300 |
| Components | High Fidelity PCR Master mix containing enzyme blend, reaction buffer, and nucleotides (10 vials); Water, PCR Grade (5 vials) |