Anti-HA-Biotin, High Affinity (3F10)
ROCHE/12158167001 - from rat IgG1
Synonym: antibody
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 3F10, monoclonal |
| conjugate | biotin conjugate |
| epitope sequence | YPYDVPDYA |
| form | lyophilized (stabilized) |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 μg |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Anti-HA-Biotin, High Affinity (3F10) is used for the detection of HA-tagged recombinant proteins using: • Dot blots • ELISA (enzyme-linked immunosorbent assay) • Western blots Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling. It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay. |
| General description: | Anti-HA-Biotin, High Affinity (3F10) is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to biotin. The Anti-HA-Biotin, High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5. It is a monoclonal antibody whose high affinity and low working concentrations result in less cross-reactivity than with other antibodies to the HA-epitope. Anti-HA-Biotin, High Affinity (3F10) is a biotin conjugate of this clone which is specifically useful in western blotting, ELISA applications and assays using the universal biotin-streptavidin platform, by allowing specific and highly sensitive detection of HA-tagged proteins. |
| Immunogen: | Amino acids 98-106 from the human influenza virus hemagglutinin protein |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Sample Materials Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines: • Bacterial extracts: 20 mM Tris, pH 8.0, 100 mM NaCl, cOmplete Protease Inhibitor Cocktail Tablets, followed by freeze-thaw. • Mammalian extracts: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.1% Nonidet P40, complete Protease Inhibitor Cocktail Tablets. • Other cell lysis buffers may be more appropriate for individual applications. In general, to obtain optimal performance of the affinity matrix: • Use protease inhibitors to reduce proteolytic activity. Use complete Protease Inhibitor Cocktail Tablets for most applications. • Limit detergent to the lowest concentration levels necessary to obtain adequate cell lysis. |
| Preparation Note: | Working concentration: Working concentration of conjugate depends on application and substrate The following concentrations should be taken as a guideline:• Dot blot: 100 ng/ml • ELISA: 100 ng/ml • Western blot: 100 ng/ml |
| Quality: | Function test: The Anti-HA-Biotin; High Affinity is function tested by Western blot analysis of a HA-tagged fusion protein. |
| Reconstitution: | Add 1 ml double-distilled water to a final concentration of 50 μg/ml. Rehydrate for 10 minutes prior to use. |
| Specificity: | Anti-HA-Biotin, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P273 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |