3-Hydroxybutyrate Dehydrogenase (3-HBDH)
ROCHE/3HBDB-RO - suspension, ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.), grade II
Synonym: 3-Hydroxybutyrate Dehydrogenase (3-HBDH); 3-HBDH
Product Type: Chemical
| concentration | 5 mg/mL |
| form | suspension |
| impurities | <0.1% LDH |
| <5% MDH | |
| manufacturer/tradename | Roche |
| optimum pH | 6.2-6.9(for reduction) |
| 7-9(for oxidation) | |
| packaging | vial of 2 mL (10127833001) |
| vial of 5 mL (10127841001) | |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.) |
| storage temp. | 2-8°C |
| Analysis Note: | Contaminants: <0.1% LDH, <5% MDH |
| Application: | 3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxyl |
| Biochem/physiol Actions: | 3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples. |
| General description: | 3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Suspension in 3.2 M ammonium sulfate solution, pH approximately 6 |
| Preparation Note: | Stabilizers: NADH, Ca2+ |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| activity | specific activity: ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.30 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |