Advanced Search



BCIP

ROCHE/BCIPP-RO - 99% (HPLC), powder, solubility: in DMF, soluble

Synonym: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; BCIP® p-toluidine salt; X-phosphate p-toluidine salt

CAS Number: 6578-06-9
Empirical Formula (Hill Notation): C8H6BrClNO4P · C7H9N
Molecular Weight: 433.62
MDL Number: MFCD00040636
Linear Formula: C8H6BrClNO4P · C7H9N
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-10760994001 250 mg
 $205.00
1/EA		 Add To Favorites
45-11585002001 1 g
 $445.00
1/EA		 Add To Favorites

 

assay 99% (HPLC)
description 5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt
form powder
InChI 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key QEIFSLUFHRCVQL-UHFFFAOYSA-N
manufacturer/tradename Roche
mol wt Mr 326.44 (BCIP)
  Mr 433.6 (BCIP toluidine salt)
packaging pkg of 1 g (11585002001)
  pkg of 250 mg (10760994001)
Quality Level 100 
SMILES string Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
solubility DMF: soluble
storage temp. 2-8°C
Analysis Note: Absorption: Absorption maximum in the visible light is not determined.
Application: For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
General description: BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.
Legal Information: BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: For life science research only. Not for use in diagnostic procedures.
Quality: Performance test: incubation with AP
Substrates: Histochemical substrate for alkaline phosphatase.
Purity 99% (HPLC)
Storage Temp. 2-8°C
UNSPSC 12352200

The following items have been added to your cart:

Choose a favorite list for this item:
Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top