BCIP
ROCHE/BCIPP-RO - 99% (HPLC), powder, solubility: in DMF, soluble
Synonym: 5-
CAS Number: 6578-06-9
Empirical Formula (Hill Notation): C8H6BrClNO4P · C7H9N
Molecular Weight: 433.62
MDL Number: MFCD00040636
Linear Formula: C8H6BrClNO4P · C7H9N
Product Type: Chemical
assay | 99% (HPLC) |
description | 5-bromo-4-chloro-3-indoly |
form | powder |
InChI | 1S/C8H6BrClNO4P.C7H9N/c9- |
InChI key | QEIFSLUFHRCVQL-UHFFFAOYSA |
manufacturer/tradename | Roche |
mol wt | Mr 326.44 (BCIP) |
Mr 433.6 (BCIP toluidine salt) | |
packaging | pkg of 1 g (11585002001) |
pkg of 250 mg (10760994001) | |
Quality Level | 100 ![]() |
SMILES string | Cc1ccc(N)cc1.OP(O)(=O)Oc2 |
solubility | DMF: soluble |
storage temp. | 2-8°C |
Analysis Note: | Absorption: Absorption maximum in the visible light is not determined. |
Application: | For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid. |
General description: | BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected. |
Legal Information: | BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Quality: | Performance test: incubation with AP |
Substrates: | Histochemical substrate for alkaline phosphatase. |
Symbol | ![]() |
Signal word | Warning |
Hazard statements | H312 - H315 - H319 - H335 |
Precautionary statements | P261 - P264 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P337 + P313 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | 136.4 °F |
Flash Point(C) | 58 °C |
Purity | 99% (HPLC) |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |