β-Glucuronidase/Arylsulfatase
ROCHE/BGALA-RO - from Helix pomatia
Synonym: arylsulfatase/β-glucuronidase; sulfatase/β-glucuronidase
Product Type: Chemical
application(s) | life science and biopharma sample preparation |
biological source | animal (Helix pomatia) |
color | colorless to brown |
form | solution |
manufacturer/tradename | Roche |
optimum pH | 4.5-5.0 |
packaging | pkg of 10 mL (10127698001) |
pkg of 2 mL (10127060001) | |
Quality Level | 100 ![]() |
solubility | water: miscible |
specific activity | 10.8 U/mL, 25 °C (ß-Glucuronidase) |
25 U/mL, 25 °C (with 4-NP-sulfate, Arylsulfatase) | |
storage temp. | 2-8°C |
technique(s) | ELISA: suitable |
Application: | β-Glucuronidase/Arylsulfa |
Components: | EC 3.1.6.1 & 3.2.1.31 |
Features and Benefits: | • Deconjugate and detect both glucuronides and sulfate esters. • Quickly screen for steroids, benzodiazepines, cannabinoids, opioids, and other drugs. |
General description: | β-Glucuronidase/Arylsulfa |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Physical form: | Solution in saline, stabilized |
Preparation Note: | Working concentration: In many applications the product can be diluted with water immediately before use or employed undiluted. Note: The present β-glucuronidase/arylsulfa Storage conditions (working solution): Note: Aliquot portions of the diluted preparation may be stored at -15 to -25 °C; they should not be thawed and refrozen more than a time or two, and storage at the lower temperature does not lengthen their life beyond that of the product kept at 2 to 8 °C. |
Principle: | Glucuronidation is one of the basic principles of metabolism. Most substances presented to the human body undergo metabolic processing that includes conjugation with glucuronic acid by UDP-glucuronosyltransfera In addition, sulfation of the drug or chemical substance by sulfotransferases in the human body may occur. Sulfation in general, is less predictable than glucuronidation since many isoenzymes of sulfotransferases exist, and there are individual differences in the ratio of glucuronidation versus sulfation of the substance. |
Specificity: | β-Glucuronidase/Arylsulfa Specificity of β-Glucuronidase: The glycosides that β-D-glucuronic acid forms with a variety of compounds containing hydroxyl groups hydrolyze readily in the presence of β-glucuronidase. • Such compounds include steroids, such as estriol (Km = 0.42 mM, pH 4.5), androsterone, pregnanediol, tetrahydrocortisone, • phenols, such as phenolphthalein (Km = 0.39 mM), 4-nitro-phenol, 4-methylumbelliferone, • drugs such as chloramphenicol and tetrahydrocannabinols, • and metabolites such as thyroxine and bilirubin. Polysaccharides that contain β-glucuronic acid residues, such as hyaluronic acid, are also hydrolyzed. β-Glucuronidase is highly specific for the carbohydrate part: neither α-glucosides nor β-glucosiduronic acids are hydrolyzed. However, the nature of the residue linked to the β-glucuronic acid residue is hardly important at all. Specific activity: 5.5 U/ml at +38°C with phenolphthalein-β-glucuronide as the substrate (4.5 U/ml at +25°C with 4-nitro-phenyl-β-D-glucuronide as the substrate = 100,000 Fishman units/ml at +38°C with phenolphthalein-β-glucuronide as the substrate). 1 Fishman unit releases 1 μg phenolphthalein from phenolphthalein-β-glucuronide in 1 hour at +38°C. Specificity of Acrylsulfatase: Sulfate esters of many phenols are hydrolysed in the presence of arylsulfatase. Examples are steroid sulfates such as estronesulfate, 4-nitrophenyl hydrogen sulfate (Km = 1.8 mM, pH 7.3), 4-nitro-pyrocatechol 2-sulfate (Km = 1.25 mM, pH 7.5), and phenolphthalein disulfate. Specific activity: 2.6 U/ml at +38°C with phenolphthalein disulfate as the substrate (14 U/ml at +25°C with 4-nitrophenyl sulfate as the substrate = 800,000 Roy units/ml at +38°C with 2-hydroxy-5-nitrophenyl sulfate as the substrate). 1 Roy unit releases 1 μg 2-hydroxy-5-nitrophenyl sulfate in 1 hour at +38°C. |
Specificity: | Specificity of β-Glucuronidase: The glycosides that β-D-glucuronic acid forms with a variety of compounds containing hydroxyl groups hydrolyze readily in the presence of β-glucuronidase. • Such compounds include steroids, such as estriol (Km = 0.42 mM, pH 4.5), androsterone, pregnanediol, tetrahydrocortisone, • phenols, such as phenolphthalein (Km = 0.39 mM), 4-nitro-phenol, 4-methylumbelliferone, • drugs such as chloramphenicol and tetrahydrocannabinols, • and metabolites such as thyroxine and bilirubin. Polysaccharides that contain β-glucuronic acid residues, such as hyaluronic acid, are also hydrolyzed. β-Glucuronidase is highly specific for the carbohydrate part: neither α-glucosides nor β-glucosiduronic acids are hydrolyzed. However, the nature of the residue linked to the β-glucuronic acid residue is hardly important at all. Specificity of Acrylsulfatase: Sulfate esters of many phenols are hydrolysed in the presence of arylsulfatase. Examples are steroid sulfates such as estronesulfate, 4-nitrophenyl hydrogen sulfate (Km = 1.8 mM, pH 7.3), 4-nitro-pyrocatechol 2-sulfate (Km = 1.25 mM, pH 7.5), and phenolphthalein disulfate. |
Storage and Stability: | Avoid repeated freezing and thawing (more than 2 times). |
Unit Definition: | Glucuronidase: Standard unit The standard unit of β-glucuronidase activity is the enzyme activity that increases the rate of release of 4-nitrophenol from 4-nitrophenyl β-D-glucosiduronic acid at a temperature of +25 °C and pH 4.5 by 1 μM. Phenolphthalein unit The phenolphthalein unit of β-glucuronidase activity is the enzyme activity that increases the rate of release of phenolphthalein from phenolphthalein β-D-glucosiduronic acid at a temperature of +38 °C by 1 μM. Approx. 4.5 standard units are equivalent to 5.5 phenolphthalein units. Fishman unit The Fishman unit of β-glucuronidase activity is the enzyme activity that increases the rate of release of phenolphthalein from phenolphthalein β-D-glucosiduronic acid at a temperature of +38 °C by 1 μg. Approx. 1 standard unit is equivalent to 22,000 Fishman units (1 phenolphthalein unit is equivalent to 19,000 Fishman units). Arylsulfatase: Standard unit The standard unit of arylsulfatase activity is the enzyme activity that increases the rate of release of 4-nitrophenol from 4-nitrophenyl sulfate at a temperature of +25 °C and pH 6.2 by 1 μM. Phenolphthalein unit The phenolphthalein unit of arylsulfatase activity is the enzyme activity that increases the rate of release of phenolphthalein from phenolphthalein disulfate at a temperature of +38 °C and pH 6.2 by 1 μM. Approx. 5.4 standard units are equivalent to 1 phenolphthalein unit. Roy unit The Roy unit of arylsulfatase activity is the enzyme activity that increases the rate of release of 4-nitropyrocatechol from 2-hydroxy-5-nitrophenyl hydrogen sulfate (4-nitropyrocatechol 2-sulfate) at a temperature of +38 °C and pH 6.2 by 1 μg. Approx. 1 standard unit is equivalent to 57,000 Roy units (1 phenolphthalein unit is equivalent to 308,000 Roy units). |
RIDADR | NONH for all modes of transport |
WGK Germany | nwg |
Flash Point(F) | No data available |
Flash Point(C) | No data available |
activity | specific activity: 10.8 U/mL, 25 °C (ß-Glucuronidase); specific activity: 25 U/mL, 25 °C (with 4-NP-sulfate, Arylsulfatase) |
Storage Temp. | 2-8°C |
UNSPSC | 12352204 |