Advanced Search



β-Glucuronidase

ROCHE/BGALS-RO - from E. coli K 12

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-3707580001 1 mL
$91.40
1/EA
Add To Favorites
45-3707598001 5 mL
$261.00
1/EA
Add To Favorites
45-3707601001 15 mL
$602.00
1/EA
Add To Favorites
Β-Glucuronidase

 

application(s) detection
sample preparation
biological source Escherichia coli K12
color colorless
form solution
manufacturer/tradename Roche
mol wt Mr ~220 kDa
optimum pH 6.0-6.5
packaging pkg of 1 mL (03707580001)
  pkg of 15 mL (03707601001)
  pkg of 5 mL (03707598001)
parameter 48 °C optimum reaction temp.
Quality Level 100 
solubility water: soluble
storage condition (Keep container tightly closed in a dry and well-ventilated place.)
storage temp. 2-8°C
suitability suitable for enzyme test
technique(s) activity assay: suitable
UniProt accession no.  
  P05804 
Application: β-Glucuronidases have been used extensively in research and analytical laboratories for the enzymatic hydrolysis of steroid β-glucuronides. β-Glucuronidase is used:• for the hydrolysis of steroid conjugates (glucuronides) in urine (pH 6.0–6.5)
• in doping analysis
• for the detection of benzodiazepine in small doses.
• during sample preparation to cleave off glucuronides prior to GC-MS, HPLC, immunoassays, or other analytical methods.
It has been used for the hydrolysis of plasma enterolactone, testosterone and epitestosterone glucuronide.
Biochem/physiol Actions: Glucuronidation is one of the basic principles of metabolism. Many substances presented to the human body undergo metabolic processing that includes conjugation with glucuronic acid by UDP-glucuronosyltransferases (UGTs). β-Glucuronidase, also known as β-D-glucuronoside glucuronosohydrolase, catalyzes the transfer of a glucuronyl group to many biological and pharmacologically active endogenous and exogenous molecules. Glucuronide is in general, more soluble, less toxic, and more easily excreted by the human body compared to the original molecule. To analyze these drug conjugates that are present in body fluids, such as urine and plasma, deconjugation of the glucuronide is necessary. Enzymatic hydrolysis prior to detection is essential to achieving high sensitivity during analysis. After hydrolysis, the sample may be analyzed by mass spectroscopy, gas chromatography, HPLC, immunoassays, or other analytical methods.
Features and Benefits: β-Glucuronidase from E. coli is highly specific for β-glucuronides, and is capable of very quickly hydrolyzing steroid β-glucuronides as well as cleaving many other types of glucuronides, such as benzodiazepine, opioid, and cannabinoid. β-Glucuronidase is used for the enzymatic hydrolysis of glucuronides in biological fluids, primarily urine. Substances used for doping are conjugated with glucuronides, which means that effective doping analysis relies very often on the enzymatic cleavage of conjugated drug molecules by β-glucuronidase.

• Perform fast analysis due to the enzyme′s high specific activity.
• Quickly screen for steroids, benzodiazepines, cannabinoids, and opioids.
• Save time by developing your procedure without the need to clean up the reaction or buffer the urine.
General description: Glucuronidase at 25 °C, or 140 U/mg at 37 °C, at pH 7 with 4-nitrophenyl-β-D-glucuronide as substrate; 1 ml β-Glucuronidase contains at least 140 U at 37 °C.
Legal Information: The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.
Other Notes: The E.coli β-glucuronidase enzyme is essentially free of sulfatase activity and is especially efficient in cleaving steroid and benzodiazepine conjugates.
For life science research only. Not for use in diagnostic procedures.
Physical form: Solution in 50% glycerol, pH approximately 6.5
(15 ml in one bottle)
Specificity: Cleaves terminal glucuronic acid which is β-linked to mono-, oligo- or polysaccharides or phenol.
Unit Definition: The international unit of β-glucuronidase activity is the enzyme activity that increases the rate of release of 4-nitrophenol from 4-nitrophenyl-β-D-glucuronide (4NPG) at a temperature of +25 °C and pH 7.0 by 1 μM.
The Fishman unit was previously used. This unit is the release of phenolphthalein from its glucuronide (PPG). It is not possible to measure the relative activities of different preparations for steroid β-glucuronides by comparing their activities with respect to PPG. Many preparation do not catalyze the hydrolysis of PPG, 4NPG, or the various steroid β-glucuronides in urine equally. The choice of 4NPG as standard substrate is based on the following considerations:
• The Michaelis concentrations for the two substrates are similar (KM = 2 ×10-4 M for 4 NPG and KM = 6 ×10-5 M for PPG), but the corresponding rates of hydrolysis differ:
• 4NPG is hydrolysed about 5 × as fast as PPG;
• For PPG, inhibition by excess substrate occurs; this is not observed using 4NPG.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) No data available
Flash Point(C) No data available
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.2.1.31   ( BRENDA  | IUBMB  )
UNSPSC 12352204

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top