CDP-Star®
ROCHE/CDP-RO - Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate
Synonym: CDP-Star® Chemiluminescent Substrate; ready-to-use, cdp-star; Disodium 2-
CAS Number: 160081-62-9
Empirical Formula (Hill Notation): C18H19Cl2O7Na2P
Molecular Weight: 495.20
MDL Number: MFCD02094611
Linear Formula: C18H19Cl2O7Na2P
Product Type: Chemical
assay | >98% |
form | solution |
InChI | 1S/C18H21Cl2O7P.2Na/c1-24 |
InChI key | UKWLRLAKGMZXJC-VCCHNUGISA |
manufacturer/tradename | Roche |
packaging | bottle of 1 mL (11685627001[25 mM]) |
bottle of 2 × 1 mL (11759051001[25 mM]) | |
Quality Level | 100 ![]() |
shipped in | wet ice |
SMILES string | Cl[C@]1(C2)C[C@H]3C4(OOC4 |
storage temp. | 2-8°C |
Application: | Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots: • Southern blot • Northern blot • Dot blot • Colony and plaque hybridization • Gel shift assays |
Features and Benefits: | CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems. • Save time with a fast, ultra-sensitive substrate. • Easily strip and reprobe membranes. • Take multiple exposures of up to two days for each experiment. • Reuse the substrate by filtering and storing in sodium azide. |
General description: | Contents 25 mM solution (12.38 mg/ml), 100x concentration |
Legal Information: | CDP-Star is a registered trademark of Tropix, Inc. |
Other Notes: | • First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result. • No preincubation step required. • Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background). • Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film. • Exposure times are shorter than with CSPD. • Do not use nitrocellulose membranes. • Repeat exposures can be made up to two days after the addition of substrate. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Preparation Note: | Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable Maleic Acid Buffer: 15 to 25 °C, stable Detection Buffer: 15 to 25 °C, stable Blocking Solution: always prepare freshly Antibody Solution: 2 to 8 °C |
Quality: | Purity: Purified by HPLC. |
RIDADR | NONH for all modes of transport |
WGK Germany | nwg |
Flash Point(F) | No data available |
Flash Point(C) | No data available |
Purity | >98% |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |