CDP-Star^®

ROCHE/CDP-RO - Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.1^3,7 ]decan}-4-yl)-1-phenyl phosphate

Synonym: CDP-Star^® Chemiluminescent Substrate; ready-to-use, cdp-star; Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.1^3.7]decan])-4-yl]-1-phenyl phosphate

CAS Number: 160081-62-9
Empirical Formula (Hill Notation): C₁₈H₁₉Cl₂O₇Na₂P
Molecular Weight: 495.20
MDL Number: MFCD02094611
Linear Formula: C₁₈H₁₉Cl₂O₇Na₂P
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-11685627001	1 mL	$383.00 1/EA	Add To Favorites
45-11759051001	1 mL	$652.00 1/EA	Add To Favorites

CDP-Star, ready-to-use

Properties
Descriptions
Safety Info.
Basic Data

assay	>98%
form	solution
InChI	1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;2*+1/p-2/t10-,11-,12+,16-,17?,18?;;
InChI key	UKWLRLAKGMZXJC-VCCHNUGISA-L
manufacturer/tradename	Roche
packaging	bottle of 1 mL (11685627001[25 mM])
	bottle of 2 × 1 mL (11759051001[25 mM])
Quality Level	100
shipped in	wet ice
SMILES string	Cl[C@]1(C2)C[C@H]3C4(OOC4(C5=CC(OP([O-])([O-])=O)=C(Cl)C=C5)OC)[C@@H]2C[C@@H](C1)C3
storage temp.	2-8°C

Application:	Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots: • Southern blot • Northern blot • Dot blot • Colony and plaque hybridization • Gel shift assays
Features and Benefits:	CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems. • Save time with a fast, ultra-sensitive substrate. • Easily strip and reprobe membranes. • Take multiple exposures of up to two days for each experiment. • Reuse the substrate by filtering and storing in sodium azide.
General description:	Contents 25 mM solution (12.38 mg/ml), 100x concentration
Legal Information:	CDP-Star is a registered trademark of Tropix, Inc.
Other Notes:	• First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result. • No preincubation step required. • Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background). • Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film. • Exposure times are shorter than with CSPD. • Do not use nitrocellulose membranes. • Repeat exposures can be made up to two days after the addition of substrate.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Preparation Note:	Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable Maleic Acid Buffer: 15 to 25 °C, stable Detection Buffer: 15 to 25 °C, stable Blocking Solution: always prepare freshly Antibody Solution: 2 to 8 °C
Quality:	Purity: Purified by HPLC.

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point(F)	No data available
Flash Point(C)	No data available

Purity	>98%
Storage Temp.	2-8°C
UNSPSC	12352200

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