Advanced Search



Cell Death Detection ELISAPLUS

ROCHE/CELLDETH-RO - sufficient for 96 multiwell tests (11774425001), sufficient for 10 x 96 multiwell tests (11920685001)

Synonym: ELISA

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11920685001 95 tests
$3460.00
1/EA
Add To Favorites
45-11774425001 96 tests
$718.00
1/EA
Add To Favorites
Cell Death Detection ELISAPLUS
Schematic showing the principle of the Cell Death Detection ELISAPLUS.
Dose-dependent induction of apoptosis in U937 cells, detected using the Cell Death Detection ELISAPLUS U937 cells (104 cells/well, in 200 μL) were incubated with different concentrations of camptothecin (CAM) for 4 hours at +37 °C. Before and after lysis, cells were centrifuged and the supernatant was analyzed. Results were plotted as dose vs. response. Result: Amounts of cytoplasmic oligonucleosomes (an indicator of apoptosis) increase as CAM concentration increases. Cell culture supernatants removed from the cells after treatment (but before lysis) gave no signal, indicating that there were no necrotic cells during the treatment.

 

detection method photometric
manufacturer/tradename Roche
Quality Level 100 
storage temp. 2-8°C
technique(s) ELISA: suitable
usage sufficient for 10 x 96 multiwell tests (11920685001)
  sufficient for 96 multiwell tests (11774425001)
Application: For research use only. Not for use in diagnostic procedures.

The Cell Death Detection ELISAPLUS photometric enzyme immunoassay is used for the quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell death.
The kit contains a stop solution which allows users to terminate the substrate reaction and run the assay under defined conditions, making it suitable for use in high-throughput applications.
Features and Benefits: • One-step ELISA
• High sensitivity: (5 x 102 cells/ml)
• Fast performance: (3 - 4 hours)
• Positive control included
• No prelabeling of cells necessary
• Nonradioactive assay system
• No species restriction
• Easy handling
• Low background
• Suppressed human anti-mouse factor
• Function tested
General description: Use this kit for relative quantification of histone-complexed DNA fragments (mono- and oligonucleosomes) out of the cytoplasma of cells after the induction of apoptosis or when released from necrotic cells. Since the assay does not require prelabeling of cells, it can detect internucleosomal degradation of genomic DNA during apoptosis even in cells that do not proliferate in vitro, for example, freshly isolated tumor cells. The antibodies used in the assay are not species-specific, therefore, the kit may be used to assay cells from a wide variety of species.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Packaging: • 11774425001 (1 kit containing 10 components)
• 11920685001 (1 kit containing 10 components)

Packaging: 1 kit containing 10 components
Preparation Note: Working solution: Preparation of Working Solutions

Alongside the ready-to-use solutions supplied with this kit, you will need to prepare the following working solutions:
Note: Use only double-distilled water for reconstitution of lyophilizates.

Anti-Histone Biotin
Reconstitute the lyophilizate in 450 μl double-distilled water for 10 minutes and mix thoroughly.
Use: Component of the Immunoreagent.
Anti-DNA Peroxidase
Reconstitute the lyophilizate in 450 μl double-distilled water for 10 minutes and mix thoroughly.
Use: Component of the Immunoreagent.
Positive Control
Reconstitute the lyophilizate in 450 μl double-distilled water for 10 minutes and mix thoroughly.
Use: ELISA Step 1.
ABTS Tablets
Dependent on the number of samples tested, dissolve 1, 2, or 3 tablets from bottle 7 in 5, 10, or 15 ml Substrate Buffer.
Use: ELISA Step 5.
ABTS Stop Solution
If turbidity or a precipitate is visible, warm to 37 °C with shaking until the solution is clear.
Use: ELISA Step 6.
Storage conditions (working solution): Anti-Histone Biotin: 2 to 8 °C for 2 months
ABTS Tablets: 1 month stored protected from light. Allow to come to 15 to 25 °C before use.
ABTS Stop Solution: 2 to 8 °C until the expiration date printed on the label.
Preparation Note: The exact detection limit of dying/dead cells in a particular sample strongly depends on the kinetics of cell death, the cytotoxic agent used, and the amount of affected cells in the total cell population. Using U937/camptothecin (CAM) as a cellular model system for cell death, the immunoassay allows the specific detection of mono- and oligonucleosomes in the cytoplasmic fraction of 125cell equivalents/well.
Specifications: Assay time: 3 - 4 hours
Negative control: Depending on cell culture conditions, each exponentially growing permanent cell culture contains a certain amount of dead cells (typically approximately 3 - 8%). In the immunoassay, these inherent dead cells in the untreated sample (without a cell-death-inducing agent) will cause a certain absorbance value (negative control).
Positive control: A DNA-histone complex serves as a positive control.
Sample material: Cytoplasmic fractions (lysates) of cell lines, cells ex vivo, cell culture supernatants, and serum or plasma
Specificity: Anti-histone reacts with the histones H1, H2A, H2B, H3, and H4 of various species (e.g., human, mouse, rat, hamster, cow, opossum, Xenopus). Anti-DNA binds to single- and double-stranded DNA. Therefore, the ELISA allows the detection of mono- and oligonucleosomes from various species, and may be applied to measure apoptotic cell death in many different cell systems.
Unit Definition: Unit Conversion: 1 mU = 1 x 10-3 OD (1 mU = 0.001 OD).

Storage Temp. 2-8°C
UNSPSC 41116158
Components Anti-histone-biotin antibody (clone H11-4); Anti-DNA-POD antibody (clone MCA-33); Positive Control; Incubation Buffer; Lysis Buffer; Substrate Buffer; ABTS Substrate Tablet; ABTS Stop Solution; Microplate (streptavidin-coated); Adhesive Cover Foils

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top