Collagenase A
ROCHE/COLLA-RO - from Clostridium histolyticum
Product Type: Chemical
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| application(s) | cell analysis life science and biopharma sample preparation |
| biological source | Clostridium histolyticum |
| color | brown |
| concentration | 1 mg/mL (0.1%, w/v) |
| foreign activity | Clostripain 9.489 U/mg |
| Proteases 6.067 U/mg (Azocoll ) | |
| Trypsin 0.144 U/mg (with BAEE) | |
| form | lyophilized |
| manufacturer/tradename | Roche |
| NCBI accession no. | WP_138210331   |
| optimum pH | 6.0-8.0 |
| packaging | pkg of 100 mg (10103578001) |
| pkg of 2.5 g (11088793001) | |
| pkg of 500 mg (10103586001) | |
| Quality Level | 100 |
| solubility | water: soluble |
| specific activity | >0.15 U/mg lyophilizate (Collagenase activity) |
| sterility | non-sterile |
| storage temp. | 2-8°C |
| suitability | suitable for PCR |
| UniProt accession no. | A0A4U9RG91 |
| Application: | Collagenase from C. histolyticum is widely used for the: • Disaggregation of many types of tissues (e.g., lung, heart, muscle, bone, adipose tissue, liver, kidney, cartilage, mammary gland, placentae, blood vessels, brain, tumors, umbilical cord) • preparation of single-cell suspensions for the establishment of primary cell culture systems. Collagenase A is recommended when yield, viability, and functionality are important. • preparation of cells from many types of tissue, such as hepatocytes, adipocytes, pancreatic islets, epithelial cells, muscle cells, endothelial cells, etc. However, the suitability of each lot of the enzyme for disruption of a particular tissue should be determined empirically. |
| General description: | Collagenase is a protease which cleaves the triple-helical protein called collagen. Clostridium histolyticum produces six types of collagenases namely, α, β, γ, δ, ε and λ. These collagenases are active against connective tissue and show more reactivity towards gelatin than collagen. Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10 °C. |
| Other Notes: | Shipping conditions may vary |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: Ca2+ Inhibitors: Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercapto-ethanol Collagenase is not inhibited by serum. Clostripain inhibitors: TLCK Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean), serum Working concentration: 0.5 to 2.5 mg/ml Storage conditions (working solution): -15 to -25 °C Roche recommends reconstituting only the amount of lyophilizate needed for immediate use. The reconstituted solution can be stored at -15 to -25 °C for up to one week. Avoid repeated freezing and thawing since activity decreases after reconstitution. |
| Reconstitution: | Reconstitution in any balanced salt solution (e.g., HBSS) |
| Specificity: | Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins. Enzyme activity: Collagenase activity: >0.15U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycar Contaminating enzyme activities: trypsin, clostripain, and neutral proteolytic activity Collagenase A has a balanced ratio of enzyme activities. |
| Storage and Stability: | Keep container tightly closed in a dry and well-ventilated place |
| Unit Definition: | Contents Lyophilizate, nonsterile |
| Unit Definition: | Unit Definition: Collagenase from Roche is assayed in Wünsch units (1 μmol of product formed per minute at +25 °C with Wünsch substrate). Frequently, collagenase activities are given in Mandl units (1 μmol leucine liberated from collagen in 5 hours at +37 °C). Unfortunately, there is no consistent conversion factor between the two units of activity, since the Mandl unit depends, in part, on the concentration of contaminating proteases in the collagenase preparation, an indefinable variable. A purer collagenase preparation would actually give a lower specific activity in Mandl units than a crude preparation. Clostridium preparations typically give conversion factors of approximately 1:1800 (e.g., a particular lot of Clostridium collagenase contained approximately 0.15 Wünsch U/mg and 250 Mandl U/mg). |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: >0.15 U/mg lyophilizate (Collagenase activity) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.24.3 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |