Collagenase/Dispase®
ROCHE/COLLDISP-RO - lyophilized, non-sterile, optimum pH 7.0-8.0
Synonym: collagenase
Product Type: Chemical
| application(s) | life science and biopharma sample preparation |
| biological source | enzyme from Bacillus polymyxa ( Dispase ) |
| enzyme from bacterial ( Collagenase from Vibrio alginolyticus) | |
| collagenase activity | >0.1 U/mg lyophilizate, 25 °C (4-phenyl-azobenzyl-oxyca |
| color | light yellow to brown |
| concentration | 1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL) |
| foreign activity | proteolytic activities <0.1 U/mg (hemoglobin as the substrate) |
| form | lyophilized |
| manufacturer/tradename | Roche |
| optimum pH | 7.0-8.0 |
| packaging | pkg of 100 mg (10269638001) |
| pkg of 500 mg (11097113001) | |
| Quality Level | 100  |
| solubility | water: soluble |
| specific activity | >0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase) |
| sterility | non-sterile |
| storage temp. | 2-8°C |
| technique(s) | tissue processing: suitable |
| UniProt accession no. | P43154  |
| Application: | Collagenase/Dispase® is used for the preparation of cells from a wide variety of different tissues and organs. |
| Components: | EC 3.4.24.4 & 3.4.24.3 |
| General description: | Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact. Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10 °C. |
| Legal Information: | Dispase is a registered trademark of Godo Shusei Co., Ltd. |
| Other Notes: | Collagenase from Vibrio alginolyticus, Dispase® from Bacillus polymyxa |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: Ca2+ Working concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL) Enzyme activity: Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycar Dispase®: 0.8 Wünsch U/mg lyophilizate (+37°C, casein as substrate, pH 7.5) Contaminating proteolytic activities: <0.1U/mg lyophilizate (hemoglobin as the substrate) Working solution: Preparation of working solution Dissolve enzyme in water to a final concentration of 100mg/mL. Dilute with PBS (Ca2+/Mg2+-free) to a final concentration of 1mg/mL and filter through a sterile 0.2μm membrane filter. Enzyme concentration of the sterile solution: Collagenase, 0.1U/mL PBS; Dispase®, 0.8U/mL PBS. Inhibitors: Collagenase inhibitors: EDTA, EGTA Collagenase/Dispase is not inhibited by serum. Storage conditions (working solution): -15 to -25°C The reconstituted solution is stable at -15 to -25°C. Note: Store in appropriate aliquots and avoid repeated freezing and thawing! |
| Reconstitution: | Reconstitution in water (100mg/mL). Further dilution with PBS (phosphate buffered saline) (Ca2+/Mg2+-free). |
| Specificity: | Collagenase degrades native collagen. Dispase® is a nonspecific protease. |
| Storage and Stability: | Store dry |
| Unit Definition: | One unit is the enzyme activity, which liberates under assay conditions within 1 minute Folin-positive amino acids and peptides corresponding to 1 μmol tyrosine. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | collagenase activity: >0.1 U/mg lyophilizate, 25 °C (4-phenyl-azobenzyl-oxyca |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |