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Collagenase H

ROCHE/COLLH-RO - from Clostridium histolyticum

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11074032001 100 mg
$137.00
1/EA
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45-11074059001 500 mg
$513.00
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45-11087789001 2.5 g
$1860.00
1/EA
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application(s) life science and biopharma
biological source Clostridium histolyticum
collagenase activity 0.15 U/mg lyophilizate
color brown
concentration ~1 mg/mL (Isolation of rat hepatocytes)
  ~2 mg/mL (Isolation of adipocytes)
foreign activity Clostripain 39.798 U/mg
  Proteases 14.014 U/mg (Azocoll, based on lyoph)
  Trypsin 1.668 U/mg (with BAEE)
form lyophilized
manufacturer/tradename Roche
NCBI accession no. WP_138210331 
optimum pH 6.0-8.0
packaging pkg of 100 mg (11074032001)
  pkg of 2.5 g (11087789001)
  pkg of 500 mg (11074059001)
Quality Level 100 
solubility water: soluble
sterility non-sterile
storage temp. 2-8°C
technique(s) tissue processing: suitable
UniProt accession no. Q46085  
Application: Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Collagenase H is well suited for the isolation of hepatocytes from rat liver by the collagenase perfusion method. This enzyme preparation is also suitable for the preparation of other types of cells such as endothelial cells from large vessels, and for the isolation of adipocytes from epididymal fat pads of rats.
General description: Collagenase H is an enzyme mixture prepared from Clostridium histolyticum cultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Physical form: Lyophilizate, nonsterile
Preparation Note: Enzyme activity:
Collagenase activity: >0.15U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate).
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase H has a balanced ratio of enzyme activities, and is function tested for the isolation of rat hepatocytes (perfusion method).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Activator: Ca2+
Working concentration: 0.5 to 2.5mg/ml,
Approximately 1 mg/ml for the isolation of rat hepatocytes and 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution should be stored at -15 to -25°C for short term storage, -60°C or as described below for long-term storage.
Reconstitution: Reconstitution in any balanced salt solution (e.g., HBSS)
Specificity: Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins.
Storage and Stability: Keep container tightly closed in a dry and well-ventilated place.
activity collagenase activity: 0.15 U/mg lyophilizate
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.4.24.3   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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