Collagenase H
ROCHE/COLLH-RO - from Clostridium histolyticum
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | Clostridium histolyticum |
| collagenase activity | 0.15 U/mg lyophilizate |
| color | brown |
| concentration | ~1 mg/mL (Isolation of rat hepatocytes) |
| ~2 mg/mL (Isolation of adipocytes) | |
| foreign activity | Clostripain 39.798 U/mg |
| Proteases 14.014 U/mg (Azocoll, based on lyoph) | |
| Trypsin 1.668 U/mg (with BAEE) | |
| form | lyophilized |
| manufacturer/tradename | Roche |
| NCBI accession no. | WP_138210331   |
| optimum pH | 6.0-8.0 |
| packaging | pkg of 100 mg (11074032001) |
| pkg of 2.5 g (11087789001) | |
| pkg of 500 mg (11074059001) | |
| Quality Level | 100 |
| solubility | water: soluble |
| sterility | non-sterile |
| storage temp. | 2-8°C |
| technique(s) | tissue processing: suitable |
| UniProt accession no. | Q46085 |
| Application: | Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Collagenase H is well suited for the isolation of hepatocytes from rat liver by the collagenase perfusion method. This enzyme preparation is also suitable for the preparation of other types of cells such as endothelial cells from large vessels, and for the isolation of adipocytes from epididymal fat pads of rats. |
| General description: | Collagenase H is an enzyme mixture prepared from Clostridium histolyticum cultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Lyophilizate, nonsterile |
| Preparation Note: | Enzyme activity: Collagenase activity: >0.15U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycar Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity Collagenase H has a balanced ratio of enzyme activities, and is function tested for the isolation of rat hepatocytes (perfusion method). Inhibitors: Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol Collagenase is not inhibited by serum. Clostripain inhibitors: TLCK Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean) Activator: Ca2+ Working concentration: 0.5 to 2.5mg/ml, Approximately 1 mg/ml for the isolation of rat hepatocytes and 2mg/ml for the isolation of adipocytes. Storage conditions (working solution): -15 to -25°C The reconstituted solution should be stored at -15 to -25°C for short term storage, -60°C or as described below for long-term storage. |
| Reconstitution: | Reconstitution in any balanced salt solution (e.g., HBSS) |
| Specificity: | Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins. |
| Storage and Stability: | Keep container tightly closed in a dry and well-ventilated place. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | collagenase activity: 0.15 U/mg lyophilizate |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.24.3 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |