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Collagenase P

ROCHE/COLLP-RO - from Clostridium histolyticum

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11213857001 100 mg
$144.00
1/EA
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45-11213865001 500 mg
$440.00
1/EA
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45-11249002001 1 g
$733.00
1/EA
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45-11213873001 2.5 g
$1650.00
1/EA
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application(s) life science and biopharma
biological source Clostridium histolyticum
collagenase activity >1.5 U/mg (Lyophilizate, Collagenase activity)
color light brown to brown
concentration 0.5-2.5 mg/mL
foreign activity Clostripain 24.099 U/mg
  Protease 4.4 U/mg (Proteases)
  Trypsin 53.428 U/mg (with BAEE)
form lyophilized
manufacturer/tradename Roche
NCBI accession no. WP_138210331 
packaging pkg of 1 g (11249002001)
  pkg of 100 mg (11213857001)
  pkg of 2.5 g (11213873001)
  pkg of 500 mg (11213865001)
Quality Level 100 
solubility water: soluble
sterility non-sterile
storage temp. 2-8°C
technique(s) tissue processing: suitable
Application: Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Because of its high collagenase activity Collagenase P is particularly suitable for the isolation of pancreatic islets from mouse and rat (function tested). The preparation is also suitable for the isolation of adipocytes from epididymal fat pads of rats.
Features and Benefits: Contents:
Lyophilizate, nonsterile
General description: Collagenase P is an enzyme mixture prepared from the extracellular culture filtrate of a special Clostridium histolyticum strain.Collagenase P is a research biochemical, not manufactured under the US good manufacturing practices (GMP) regulations.
Note: Collagenase P is a non-sterile preparation of the culture supernatant of Clostridium histolyticum. It is neither designed nor intended for isolation of pancreatic islets for transplantation into humans and must not be used for such purposes.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Activator: Ca2+
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Working concentration: 0.5 to 2.5mg/ml
0.5 to 1.5mg/ml for the isolation of pancreatic islets from mouse and rat pancreas.
Approximately 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C. Prepare appropriate aliquots and avoid repeated freezing and thawing.
Reconstitution: Reconstitution in any balanced salt solution (e.g., HBSS)
Specificity:
Storage and Stability: Store dry
activity collagenase activity: >1.5 U/mg (Lyophilizate, Collagenase activity)
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.4.24.3   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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