CSPD ready-to-use
ROCHE/CSPD-RO - Disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate
Synonym: ready-to-use, cspd
Product Type: Chemical
| assay | >98% (HPLC) |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | Mr 461 |
| packaging | pkg of 1 mL (11655884001[25mM]) |
| pkg of 2 × 50 mL (11755633001[0.25mM] ready-to-use) | |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | CSPD ready-to-use has been used for: • the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot) • colony and plaque hybridization • gel shift assay • sequencing It may be used for the detection of alkaline phosphatase and alkaline phosphatase conjugates either in solution or on solid supports. |
| Features and Benefits: | • Save time with a fast, highly sensitive substrate. • Easily strip and reprobe membranes. • Take multiple exposures of up to two days for each experiment. • Reuse the substrate by filtering and storing in sodium azide. |
| General description: | CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. Purity: Purified by HPLC Development time: Variable Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result. Activated by preincubation (10 minutes at +37°C). Apply substrate quickly to avoid drying of membrane (background). Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film. Do not use nitrocellulose membranes. Repeat exposures can be made several days after addition of substrate. |
| Physical form: | 25mM solution (11.6mg/ml), 100x concentrated, colorless solution. |
| Preparation Note: | Storage conditions (working solution): Washing Buffer: 15 to 25 °C Maleic Acid Buffer: 15 to 25 °C Detection Buffer: 15 to 25 °C Blocking Stock Solution, 10x: 2 to 8 °C Blocking Solution: always prepare fresh Antibody Solution: 2 to 8 °C |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | >98% (HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |