dGTP
ROCHE/DGTP-RO - PCR Grade, sodium salt
Synonym: 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution; dGTP-Na3
CAS Number: 93919-41-6
Empirical Formula (Hill Notation): C10H13N5Na3O13P3
Molecular Weight: 573.13
MDL Number: MFCD00080337
Linear Formula: C10H13N5Na3O13P3
Product Type: Chemical
assay | 99% (dNTP) |
concentration | <0.9% (dNDP) |
100 mM | |
form | solution |
InChI | 1S/C10H16N5O13P3.3Na/c11- |
InChI key | IWGGLKOTEOCWQP-BIHLCPNHSA |
manufacturer/tradename | Roche |
packaging | pkg of 1,250 μL (11969030001 [100 mM]) |
pkg of 250 μL (11934538001 [100 mM]) | |
pkg of 4 × 1,240 μL (03732703001 [4x100 mM]) | |
Quality Level | 100 |
SMILES string | [Na+].[Na+].[Na+].NC1=Nc2 |
storage temp. | −20°C |
usage | sufficient for 125,000 standard reactions (03732703001) |
sufficient for 31,250 standard reactions (11969030001) | |
sufficient for 6,250 standard reactions (11934538001) |
Application: | 2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques. |
General description: | 2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5’-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase. |
General description: | dGTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosph |
Other Notes: | For general laboratory use. |
Physical form: | solution of dGTP in water (pH 8.3). |
Preparation Note: | Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade). |
Quality: | Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Purity | 99% (dNTP) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |