Advanced Search



Digoxigenin-11-dUTP, alkali-labile

ROCHE/DIUTP-RO - =85% (HPLC), solution, pkg of 25 μL (11573152910 [1 mM]), pkg of 125 μL (11573179910 [1 mM

Synonym: Digoxigenin-11-dUTP, alkali-labile; digoxigenin

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11573152910 25 µL
$335.00
1/EA
Add To Favorites
45-11573179910 125 µL
$1290.00
1/EA
Add To Favorites

 

assay 85% (HPLC)
form solution
manufacturer/tradename Roche
packaging pkg of 125 μL (11573179910 [1 mM]]])
  pkg of 25 μL (11573152910 [1 mM])
Quality Level 100 
storage temp. −20°C
Application: Digoxigenin-11-dUTP, alkali-labile can be used for non-radioactive DNA labeling e.g. random primed or nick translation. It can also be used for labeling probes which are preferred in hybridization experiments where stripping and reprobing of the membrane is intended.
Biochem/physiol Actions: Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphophate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.
General description: Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP), alkali-labile is provided as in 1 mM tetralithium salt, solution. In which DIG is bound to dUTP via an alkali-sensitive ester linkage.
Other Notes: For life science research only. Not for use in diagnostic procedures.
When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will be removed. It is therefore ideal for use in DIG-labeled probes that can be removed by alkali denaturation (e.g ., in stripping and reprobing of blots). However, if you intend to make a DIG-labeled DNA that will survive alkaline treatment, do not use this alkali-labile preparation. Instead, use the alkali-stable form of DIG-11-dUTP.
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) does not flash
Flash Point(C) does not flash
Purity 85% (HPLC)
Storage Temp. −20°C
UNSPSC 12352200

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top