T4 DNA Ligase
ROCHE/DNALIG-RO - solution, from Escherichia coli(NM 989), suitable for activity assay
Synonym: dna ligase, t4
Product Type: Chemical
| biological source | Escherichia coli (NM 989) |
| description | recombinant form of the enzyme from T4 phage |
| form | solution |
| manufacturer/tradename | Roche |
| optimum pH | 7.2-7.8 |
| packaging | pkg of 100 U (10481220001 [1 U/μl]) |
| pkg of 500 U (10716359001 [1 U/μl]) | |
| pkg of 500 U (10799009001 [5 U/μl]) | |
| parameter | 4-22 °C optimum reaction temp. |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| specific activity | ~3000 units/mg protein |
| storage temp. | −20°C |
| technique(s) | activity assay: suitable |
| Application: | Ligation of sticky- and blunt-ended DNA fragments. |
| General description: | T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase. Contents T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: Mg2+ (10mM), DTT Working solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C). |
| Quality: | Quality Tested for the absence of deoxyribonucleases and exonucleases, according to the current Quality Control procedures. |
| Specificity: | Heat inactivation: The enzyme can be inactivated by heating (65 °C, 10 minutes). |
| Unit Definition: | One unit of T4 DNA ligase is the amount of enzyme activity that converts 1 nmol 32P from pyrophosphate into Norit-absorbable material in 20 minutes at +37 °C. One unit corresponds to 0.2 U determined in the exonuclease III resistance assay. Dilution buffer: 50 mM Tris-HCl, 10 mM dithioerythritol, bovine serum albumin, 500 μg/ml, pH 7.6 (+25 °C). Volume Activity: 1 x 103 U/ml; 5 x 103 U/ml |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~3000 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |