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PCR Nucleotide Mix

ROCHE/DNTPM-RO - solution, sufficient for 500 reactions (11581295001), sufficient for 5,000 reactions (11814362001), sufficient for 2,500 reactions (04638956001)

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11581295001 200 µL
$84.40
1/EA
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45-4638956001 200 µL
$348.00
1/EA
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45-11814362001 200 µL
$440.00
1/EA
Add To Favorites
PCR Nucleotide Mix
Stability of Roche nucleotides. PCR Nucleotide Mix was exposed to 25 thermal cycles. Afterwards, enzyme, template, and primers were added, and PCR was performed for an additional 25 cycles. Lanes 1. control reaction using 1 ng λDNA 2. molecular weight marker 3. 1 ng λDNA 4. 0.1 ng λDNA 5. 0.01 ng λDNA 6. molecular weight marker Result: The intensities of the product bands in lanes 1 and 3 are equal. This indicates that there was sufficient nucleoside triphosphate in the mix to support efficient PCR, even after the nucleotides were pretreated for 25 thermal cycles.

 

assay 99.8% (sum of 4 peaks, HPLC)
color colorless
concentration 10 mM
form solution
grade Molecular Biology
impurities DNase, none detected
  RNase, none detected
manufacturer/tradename Roche
packaging pkg of 10 × 200 μL (11814362001 [10mM, each])
  pkg of 200 μL (11581295001 [10 mM, each])
  pkg of 5 × 200 μL (04638956001 [10 mM, each])
Quality Level 100 
solubility water: miscible
storage temp. −20°C
usage sufficient for 2,500 reactions (04638956001)
  sufficient for 5,000 reactions (11814362001)
  sufficient for 500 reactions (11581295001)
Application: • The PCR Nucleotide Mix is optimized for use in all types of amplification reactions and primer-extension reactions: PCR
• RT-PCR
• cDNA synthesis
• Primer extension
Features and Benefits: The PCR Nucleotide Mix can be added directly to amplification reactions. PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.

Improve yield and performance
• Choose a convenient ready-to-use mix of all 4 nucleotides.
• Achieve the highest PCR and RT-PCR sensitivity.
• Insist on highest purity (>99%) nucleotides.
General description: PCR Nucleotide Mix is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, and dTTP, each at a concentration of 10 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Packaging: 1 set containing 4 ready-to-use mixes
Preparation Note: Activator: Mg2+
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
Storage conditions (working solution): It will withstand 50 freeze/thaw cycles.
Quality: Function Testing in PCR
Function Testing in RT-PCR
Absence of Contaminating Deoxyribonucleases/Nicking Activities according to the current Quality Control procedures.
Absence of Contaminating Ribonucleases according to the current Quality Control procedures.
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) does not flash
Flash Point(C) does not flash
Purity 99.8% (sum of 4 peaks, HPLC)
Storage Temp. −20°C
UNSPSC 12352200
Components dATP, PCR Grade, sodium salt 10 mM; dCTP, PCR Grade, sodium salt 10 mM; dGTP, PCR Grade, sodium salt 10 mM; dTTP, PCR Grade, sodium salt 10 mM

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