PCR Nucleotide Mix
ROCHE/DNTPM-RO - solution, sufficient for 500 reactions (11581295001), sufficient for 5,000 reactions (11814362001), sufficient for 2,500 reactions (04638956001)
Product Type: Chemical
PCR Nucleotide Mix
Stability of Roche nucleotides. PCR Nucleotide Mix was exposed to 25 thermal cycles. Afterwards, enzyme, template, and primers were added, and PCR was performed for an additional 25 cycles. Lanes 1. control reaction using 1 ng λDNA 2. molecular weight marker 3. 1 ng λDNA 4. 0.1 ng λDNA 5. 0.01 ng λDNA 6. molecular weight marker Result: The intensities of the product bands in lanes 1 and 3 are equal. This indicates that there was sufficient nucleoside triphosphate in the mix to support efficient PCR, even after the nucleotides were pretreated for 25 thermal cycles.
| assay | 99.8% (sum of 4 peaks, HPLC) |
| color | colorless |
| concentration | 10 mM |
| form | solution |
| grade | Molecular Biology |
| impurities | DNase, none detected |
| RNase, none detected | |
| manufacturer/tradename | Roche |
| packaging | pkg of 10 × 200 μL (11814362001 [10mM, each]) |
| pkg of 200 μL (11581295001 [10 mM, each]) | |
| pkg of 5 × 200 μL (04638956001 [10 mM, each]) | |
| Quality Level | 100  |
| solubility | water: miscible |
| storage temp. | −20°C |
| usage | sufficient for 2,500 reactions (04638956001) |
| sufficient for 5,000 reactions (11814362001) | |
| sufficient for 500 reactions (11581295001) |
| Application: | • The PCR Nucleotide Mix is optimized for use in all types of amplification reactions and primer-extension reactions: PCR • RT-PCR • cDNA synthesis • Primer extension |
| Features and Benefits: | The PCR Nucleotide Mix can be added directly to amplification reactions. PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. Improve yield and performance • Choose a convenient ready-to-use mix of all 4 nucleotides. • Achieve the highest PCR and RT-PCR sensitivity. • Insist on highest purity (>99%) nucleotides. |
| General description: | PCR Nucleotide Mix is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, and dTTP, each at a concentration of 10 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 set containing 4 ready-to-use mixes |
| Preparation Note: | Activator: Mg2+ Working solution: If required, the solutions can be diluted with distilled autoclaved water. Storage conditions (working solution): It will withstand 50 freeze/thaw cycles. |
| Quality: | Function Testing in PCR Function Testing in RT-PCR Absence of Contaminating Deoxyribonucleases/Nickin Absence of Contaminating Ribonucleases according to the current Quality Control procedures. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | 99.8% (sum of 4 peaks, HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |
| Components | dATP, PCR Grade, sodium salt 10 mM; dCTP, PCR Grade, sodium salt 10 mM; dGTP, PCR Grade, sodium salt 10 mM; dTTP, PCR Grade, sodium salt 10 mM |