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DTT

ROCHE/DTT-RO - crystalline powder, =97% (Ellman′s reagent), Mr 154.3

Synonym: DL-Dithiothreitol; 1,4-dithiothreitol; cleland′s reagent; dithiothreitol, 1,4-; threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent; DTT

CAS Number: 3483-12-3
Empirical Formula (Hill Notation): C4H10O2S2
Molecular Weight: 154.25
MDL Number: MFCD00004877
Linear Formula: HSCH2CH(OH)CH(OH)CH2SH
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-10197777001 2 g
$87.80
1/EA
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45-10708984001 10 g
$247.00
1/EA
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45-11583786001 25 g
$538.00
1/EA
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assay 97% (Ellman′s reagent)
description 1,4-Dithiothreitol
form crystalline powder
InChI 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key VHJLVAABSRFDPM-QWWZWVQMSA-N
manufacturer/tradename Roche
mol wt Mr 154.3
mp 41-44 °C (lit.)
packaging pkg of 10 g (10708984001)
  pkg of 2 g (10197777001)
  pkg of 25 g (11583786001)
pH 5.1 (20 °C)
reaction suitability reagent type: reductant
SMILES string O[C@H](CS)[C@H](O)CS
storage temp. 2-8°C
Application: 1,4-dithiothreitol is used in:
• Isolation, purification and characterization of proteins and enzymes
• Measurement of enzyme activities (reactivation of enzymes)
• Determination of disulfide groups in proteins and enzymes
• DNA extraction prior to amplification
Application: An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
General description: 1,4-dithiothreitol is commonly known as Cleland′s reagent. It confers protection to thiol groups and reduces disulfide bonds in peptides and proteins. It reduces disulfide bonds to sulfhydryl group and is commonly used in receptor studies, to determine the functional importance of disulfide bonds during receptor occupancy and functionality. It also maintains monothiols completely in the reduced state.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Storage conditions (working solution): A solution of DTT in Hepes buffer, pH 7.75 is stable for one week at 2 to 8 °C if the container is tightly sealed and the solution is protected from atmospheric oxygen by argon or nitrogen.
Sequence: Enantiomers
Our DTT-preparation is optically inactive, i.e. it is the D,L-DTT.
Specifications: Oxidized form: <2.5% (absorbance at 283nm)
Purity 97% (Ellman′s reagent)
mp 41-44 °C (lit.)
Storage Temp. 2-8°C
UNSPSC 12352200

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