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dTTP

ROCHE/DTTP-RO - PCR Grade, sodium salt

Synonym: Thymidine 5′-triphosphate sodium salt solution; TTP

CAS Number: 18423-43-3
MDL Number: MFCD00080338
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11934546001 250 µL
$78.30
1/EA
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assay 99% (dNTP)
concentration <0.9% (dNDP)
  100 mM
form solution
InChI 1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
InChI key KAELIXTVSANDSE-UHFFFAOYSA-N
manufacturer/tradename Roche
packaging pkg of 1,250 μL (11969048001 [100 mM])
  pkg of 250 μL (11934546001 [100 mM])
  pkg of 4 × 1,250 μL (03732711001 [4x100 mM])
Quality Level 100 
SMILES string [Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
storage temp. −20°C
usage sufficient for 125,000 standard reactions (03732711001)
  sufficient for 31,250 standard reactions (11969048001)
  sufficient for 6,250 standard reactions (11934546001)
Application: dTTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.
General description: dTTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).
General description: PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
General description: Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.
Other Notes: For general laboratory use.
Physical form: solution of dTTP in water (pH 8.3).
Preparation Note: Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).
Quality: Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) does not flash
Flash Point(C) does not flash
Purity 99% (dNTP)
Storage Temp. −20°C
UNSPSC 12352200

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