Expand^™ High Fidelity^PLUS PCR System

ROCHE/EHIFIP-RO - sufficient for ≤500 reactions (03300226001), sufficient for ≤2,500 reactions (03300234001), suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included: no

Synonym: high fidelity pcr plus system, expand

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-3300226001	500 units	$685.00 1/EA	Add To Favorites

Comparison of Expand High FidelityPLUS PCR System to four commercially available PCR mixes. Human genomic DNA was used to amplify a 4.8 kb tPS gene fragment, using each supplier’s instructions.

Expand high fidelity plus PCR System

Properties
Descriptions
Safety Info.
Basic Data

feature	dNTPs included: no
	High Fidelity PCR
	hotstart: no
input	purified DNA
manufacturer/tradename	Roche
packaging	pkg of 2,500 U (03300234001 [10x250 U])
	pkg of 500 U (03300226001 [2x250 U])
parameter	72 °C optimum reaction temp.
Quality Level	100
storage temp.	−20°C
technique(s)	PCR: suitable
usage	sufficient for ≤2,500 reactions (03300234001)
	sufficient for ≤500 reactions (03300226001)
	sufficient for 2500 reactions
	sufficient for 500 reactions

Application:	The Expand^™ High Fidelity^PLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay. Use Expand High FidelityPLUS PCR System for: • PCR • RT-PCR • Primer extension • Carryover prevention
Features and Benefits:	• Safeguard against cross-contamination. This is the choice for large numbers of amplifications. • Obtain sixfold higher fidelity and higher yields, compared to Taq DNA Polymerase. • Efficiently label DNA. Use this kit to label DNA with radioactive or nonradioactive nucleotides.
General description:	The Expand High Fidelity^PLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity. The Expand High Fidelity^PLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone. Expand High Fidelity^PLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.
Legal Information:	Expand is a trademark of Roche
Legal Information:	Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Packaging:	1 kit containing 4 components
Quality:	Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.
Unit Definition:	Volume Activity: 5 U/μL

Hazard statements	H412
Precautionary statements	P273 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	does not flash
Flash Point(C)	does not flash

Storage Temp.	−20°C
Enzyme Commission (EC) Number	2.7.7.7 ( BRENDA \| IUBMB )
UNSPSC	41106300
Components	Expand High Fidelity^PLUS Enzyme Blend 5 U/μl; Expand High Fidelity^PLUS Reaction Buffer, with 7.5 mM MgCl₂ 5x concentrated; Expand High Fidelity^PLUS Reaction Buffer, without MgCl₂ 5x concentrated; MgCl₂ Stock Solution 25 mM

To Order From a Quote