Endoproteinase Asp-N Sequencing Grade
ROCHE/ENDOARGS-RO - from Pseudomonas fragi
Synonym: protease
Product Type: Chemical
Endoproteinase Asp-N Sequencing Grade
| assay | ≥90% (SDS-PAGE) |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | 27 kDa |
| optimum pH | 7.0-8.0 |
| packaging | pkg of 2 μg (11420488001) |
| pkg of 3 × 2 μg (11054589001) | |
| pH range | 6.5-9.0 |
| specific activity | ≥20000 units/mg protein |
| storage temp. | 2-8°C |
| Application: | Use Endoproteinase Asp-N Sequencing Grade for protein structure analysis and sequence analysis. |
| Biochem/physiol Actions: | Endoproteinase Asp-N Sequencing Grade is a metalloprotease. In phosphate, acetate, or Tris buffers at pH 6.0–8.5, it specifically cleaves peptide bonds N-terminally at aspartic and cysteic acid. If cysteine is reduced or alkylated, only -↓-Asp-X is cleaved. The specificity of Endoproteinase Asp-N Sequencing Grade is verified with glucagon as a substrate. High concentrations of Endoproteinase Asp-N Sequencing Grade (1 part by weight enzyme with 10 parts by weight glucagon) are incubated to detect traces of impurities like contaminating proteases. Heat inactivation: The protease is stopped by boiling for 5 minutes. |
| General description: | The wild type of Pseudomonas fragi segregates a metalloprotease, which cleaves peptide bonds N-terminally at small hydrophilic amino acids. A mutant of this strain produces the Endoproteinase Asp-N Sequencing Grade, which is isolated as a highly purified and specific protease. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: 2 μg /50 μl double-dist. water One vial (= 2 μg enzyme) is dissolved in 50 μl double-dist. water resulting in a buffer concentration of 10 mM Tris-HCl pH 7.5. Working solution: Recommended solvent is double-distilled water. Storage conditions (working solution): -15 to -25 °C A solution in double-dist. water is stable for 1 week at maximum at 2 to 8 °C and for about 1 month at -15 to -25 °C; repeated thawing should be avoided. |
| Preparation Note: | Lyophilized Endoproteinase Asp-N sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 10 mM Tris-HCl, pH 7.5. In order to avoid autolysis the incubation temperature should not exceed 37 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥20000 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.24.33  ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |