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Endoproteinase Asp-N Sequencing Grade

ROCHE/ENDOARGS-RO - from Pseudomonas fragi

Synonym: protease

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11420488001 2 µg
$207.00
1/EA
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45-11054589001 2 µg
$562.00
1/EA
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Endoproteinase Asp-N Sequencing Grade

 

assay ≥90% (SDS-PAGE)
form lyophilized
manufacturer/tradename Roche
mol wt 27 kDa
optimum pH 7.0-8.0
packaging pkg of 2 μg (11420488001)
  pkg of 3 × 2 μg (11054589001)
pH range 6.5-9.0
specific activity ≥20000 units/mg protein
storage temp. 2-8°C
Application: Use Endoproteinase Asp-N Sequencing Grade for protein structure analysis and sequence analysis.
General description: The wild type of Pseudomonas fragi segregates a metalloprotease, which cleaves peptide bonds N-terminally at small hydrophilic amino acids. A mutant of this strain produces the Endoproteinase Asp-N Sequencing Grade, which is isolated as a highly purified and specific protease.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Working concentration: 2 μg /50 μl double-dist. water
One vial (= 2 μg enzyme) is dissolved in 50 μl double-dist. water resulting in a buffer concentration of 10 mM Tris-HCl pH 7.5.
Working solution: Recommended solvent is double-distilled water.
Storage conditions (working solution): -15 to -25 °C
A solution in double-dist. water is stable for 1 week at maximum at 2 to 8 °C and for about 1 month at -15 to -25 °C; repeated thawing should be avoided.
Reconstitution: Lyophilized Endoproteinase Asp-N sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 10 mM Tris-HCl, pH 7.5. In order to avoid autolysis the incubation temperature should not exceed 37 °C.
Specificity: Endoproteinase Asp-N Sequencing Grade is a metalloprotease. In phosphate, acetate, or Tris buffers at pH 6.0–8.5, it specifically cleaves peptide bonds N-terminally at aspartic and cysteic acid. If cysteine is reduced or alkylated, only -↓-Asp-X is cleaved.
The specificity of Endoproteinase Asp-N Sequencing Grade is verified with glucagon as a substrate. High concentrations of Endoproteinase Asp-N Sequencing Grade (1 part by weight enzyme with 10 parts by weight glucagon) are incubated to detect traces of impurities like contaminating proteases.
Heat inactivation: The protease is stopped by boiling for 5 minutes.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) does not flash
Flash Point(C) does not flash
Purity ≥90% (SDS-PAGE)
activity specific activity: ≥20000 units/mg protein
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.4.24.33   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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