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Endoproteinase Glu-C Sequencing Grade

ROCHE/ENDOGLUS-RO - from Staphylococcus aureus V8

Synonym: protease

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11420399001 50 µg
$226.00
1/EA
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45-11047817001 50 µg
$549.00
1/EA
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Endoproteinase Glu-C Sequencing Grade
Expand Long Range dNTPack

 

biological source Staphylococcus aureus (V8)
form lyophilized
  lyophilized (salt-free)
manufacturer/tradename Roche
mol wt Mr 27 kDa
optimum pH 4.0-7.8
packaging pkg of 3 × 50 μg (11047817001)
  pkg of 50 μg (11420399001)
specific activity ≥15 units/mg protein
storage temp. 2-8°C
Application: Use Endoproteinase Glu-C Sequencing Grade for protein structure analysis and for sequence analysis.
Features and Benefits: Free of impurities that may interfere with separation of peptides in RP HPLC.
General description: Endoproteinase Glu-C Sequencing Grade is a serine protease isolated from Staphylococcus aureus V8 as a highly purified and specific protease. Inhibitors of Endoproteinase Glu-C are DFP, α2-macroglobulin and TLCK.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Working concentration: 1/100 to 1/20 (w/w)
With a minimum amount of 1 to 5 μg insulin Box, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 μm.
Working solution: Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.
Storage conditions (working solution): +2°C to +8°C for 1-2 days.
Stability: At +2 to +8°C within specification range for 12 months; stored dry.
Reconstitution: Lyophilized Endoproteinase Glu-C sequencing grade is reconstituted in double-dist. water. To avoid autolysis, the incubation temperature should not exceed 25 °C.
Specificity: Serine protease that very specifically hydrolyzes peptide bonds at the carboxylic side of Glu (in ammonium bicarbonate, pH 7.8, or ammonium acetate buffer, pH 4.0), or Glu and Asp (in phosphate buffer, pH 7.8). The specificity of Endoproteinase Glu-C Sequencing Grade is verified with the oxidized B-chain of insulin (insulin Box) as substrate. High concentrations of Endoproteinase Glu-C Sequencing Grade (1 part by weight enzyme with 10 parts by weight insulin Box) are incubated to detect traces of impurities, for example, contaminating proteases.
Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.
activity specific activity: ≥15 units/mg protein
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.4.21.19   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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