Endoproteinase Lys-C Sequencing Grade
ROCHE/ENDOLYSS-RO - from Lysobacter enzymogenes
Synonym: lys-c; lysyl endopeptidase; protease
Product Type: Chemical
| biological source | bacterial ( |
| color | white |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | 33 kDa by SDS-PAGE (reducing) |
| optimum pH | 8.5-8.8 |
| packaging | pkg of 3 × 5 μg (11047825001) |
| pkg of 5 μg (11420429001) | |
| pH range | 5-12 |
| purified by | electrophoresis |
| solubility | water: soluble |
| specific activity | ≥200 units/mg protein (at 25 °C, with Chromozym PL) |
| storage condition | ( |
| storage temp. | 2-8°C |
| suitability | suitable for enzyme test |
| technique(s) | activity assay: suitable |
| UniProt accession no. |   |
| Application: | Use Endoproteinase Lys-C Sequencing Grade for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes. |
| Biochem/physiol Actions: | This enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0). Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA. |
| General description: | Endoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease. Endoproteinase Lys-C from Lysobacter enzymogenes is a member of the peptidase S1 family. It is mostly used for the initial fragmentation of polypeptide chains in protein sequence analysis. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Storage conditions (working solution): +2 to +8 °C |
| Preparation Note: | Lyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C. Stable in 4 M urea. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ≥200 units/mg protein (at 25 °C, with Chromozym PL) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.50 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |