Endoproteinase Lys-C Sequencing Grade
ROCHE/ENDOLYSS-RO - from Lysobacter enzymogenes
Synonym: lys-c; lysyl endopeptidase; protease
Product Type: Chemical
biological source | bacterial ( |
color | white |
form | lyophilized |
grade | protein sequencing grade |
manufacturer/tradename | Roche |
mol wt | 33 kDa by SDS-PAGE (reducing) |
optimum pH | 8.5-8.8 |
packaging | pkg of 3 × 5 μg (11047825001) |
pkg of 5 μg (11420429001) | |
pH range | 5-12 |
purified by | electrophoresis |
solubility | water: soluble |
specific activity | ≥200 units/mg protein (at 25 °C, with Chromozym PL) |
storage condition | ( |
storage temp. | 2-8°C |
suitability | suitable for enzyme test |
technique(s) | activity assay: suitable |
UniProt accession no. | ![]() ![]() |
Application: | Use Endoproteinase Lys-C Sequencing Grade for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes. |
General description: | Endoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease. Endoproteinase Lys-C from Lysobacter enzymogenes is a member of the peptidase S1 family. It is mostly used for the initial fragmentation of polypeptide chains in protein sequence analysis. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Preparation Note: | Storage conditions (working solution): +2 to +8 °C |
Reconstitution: | Lyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C. Stable in 4 M urea. |
Specificity: | This enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0). Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
activity | specific activity: ≥200 units/mg protein (at 25 °C, with Chromozym PL) |
Storage Temp. | 2-8°C |
Enzyme Commission (EC) Number | 3.4.21.50 ![]() ![]() ![]() ![]() ![]() |
UNSPSC | 12352204 |