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Endoproteinase Lys-C Sequencing Grade

ROCHE/ENDOLYSS-RO - from Lysobacter enzymogenes

Synonym: lys-c; lysyl endopeptidase; protease

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11420429001 5 µg
$234.00
1/EA
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45-11047825001 5 µg
$629.00
1/EA
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biological source bacterial (Lysobacter enzymogenes)
color white
form lyophilized
grade protein sequencing grade
manufacturer/tradename Roche
mol wt 33 kDa by SDS-PAGE (reducing)
optimum pH 8.5-8.8
packaging pkg of 3 × 5 μg (11047825001)
  pkg of 5 μg (11420429001)
pH range 5-12
purified by electrophoresis
solubility water: soluble
specific activity ≥200 units/mg protein (at 25 °C, with Chromozym PL)
storage condition (Keep container tightly closed in a dry and well-ventilated place.)
storage temp. 2-8°C
suitability suitable for enzyme test
technique(s) activity assay: suitable
UniProt accession no. Q7M135 
Application: Use Endoproteinase Lys-C Sequencing Grade  for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.
General description: Endoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease. Endoproteinase Lys-C from Lysobacter enzymogenes is a member of the peptidase S1 family. It is mostly used for the initial fragmentation of polypeptide chains in protein sequence analysis.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Storage conditions (working solution): +2 to +8 °C
Reconstitution: Lyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C.
Stable in 4 M urea.
Specificity: This enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0).
Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA.
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) does not flash
Flash Point(C) does not flash
activity specific activity: ≥200 units/mg protein (at 25 °C, with Chromozym PL)
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.4.21.50   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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