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Fibronectin (pure)

ROCHE/FIBRP-RO - from human plasma, >95% (SDS-PAGE), lyophilized (clear, colorless solution after reconstitution)

Synonym: Fibronectin

MDL Number: MFCD00131062
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11051407001 1 mg
$330.00
1/EA
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45-11080938001 5 mg
$945.00
1/EA
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assay >95% (SDS-PAGE)
biological source human plasma
form lyophilized (clear, colorless solution after reconstitution)
manufacturer/tradename Roche
mol wt 440 kDa
packaging pkg of 1 mg (11051407001)
  pkg of 5 mg (11080938001)
Quality Level 100 
sterility non-sterile; 0.2 μm filtered
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
UniProt accession no. P02751 
Application: Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.
Disclaimer: RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description: Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Physical form: Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin
Preparation Note: Working concentration: 5μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Reconstitution: Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.
Sequence: Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.
Specificity: Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) does not flash
Flash Point(C) does not flash
Purity >95% (SDS-PAGE); >95% (SDS-PAGE)
Storage Temp. 2-8°C
UNSPSC 12352200

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