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Formate Dehydrogenase

ROCHE/FORMD-RO - from Candida boidinii

Synonym: FDH; Formate:NAD oxidoreductase

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-10244678001 80 units
$277.00
1/EA
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45-10837016001 250 units
$499.00
1/EA
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application(s) life science and biopharma
biological source yeast (Candida boidini)
color off-white
concentration ≥10-20 % (w/w)
foreign activity ADH <0.0500%
  LDH <0.0500%
  MDH <0.1000%
form lyophilized
manufacturer/tradename Roche
mol wt 74 kDa
optimum pH 7.5-8.5
packaging pkg of 250 U (10837016001)
  pkg of 80 U (10244678001)
Quality Level 100 
recombinant expressed in E. coli
solubility water: soluble 10 g/L
specific activity ~0.4 units/mg protein (At 25 °C with formate as the substrate.)
storage temp. 2-8°C
suitability suitable for gel electrophoresis
technique(s) activity assay: suitable
UniProt accession no. O13437 
Application: Most widely used in cofactor recycling systems for NADH.
General description: Formate:NAD+ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Quality: Contaminants: <0.005% LDH and ADH each, <0.1% MDH
Specificity: Substrate Specificity and Km:
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.
Storage and Stability: Store dry
Unit Definition: One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO2 in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.
Hazard statements H412
Precautionary statements P273 - P501
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) does not flash
Flash Point(C) does not flash
activity specific activity: ~0.4 units/mg protein (At 25 °C with formate as the substrate.)
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 1.2.1.2   ( BRENDA  | IUBMB  )
UNSPSC 12352200

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