Formate Dehydrogenase
ROCHE/FORMD-RO - from Candida boidinii
Synonym: FDH; Formate:NAD oxidoreductase
Product Type: Chemical
application(s) | life science and biopharma |
biological source | yeast (Candida boidini) |
color | off-white |
concentration | ≥10-20 % (w/w) |
foreign activity | ADH <0.0500% |
LDH <0.0500% | |
MDH <0.1000% | |
form | lyophilized |
manufacturer/tradename | Roche |
mol wt | 74 kDa |
optimum pH | 7.5-8.5 |
packaging | pkg of 250 U (10837016001) |
pkg of 80 U (10244678001) | |
Quality Level | 100 ![]() |
recombinant | expressed in E. coli |
solubility | water: soluble 10 g/L |
specific activity | ~0.4 units/mg protein (At 25 °C with formate as the substrate.) |
storage temp. | 2-8°C |
suitability | suitable for gel electrophoresis |
technique(s) | activity assay: suitable |
UniProt accession no. | O13437 ![]() ![]() |
Application: | Most widely used in cofactor recycling systems for NADH. |
General description: | Formate:NAD+ oxidoreductase Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Quality: | Contaminants: <0.005% LDH and ADH each, <0.1% MDH |
Specificity: | Substrate Specificity and Km: Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP. |
Storage and Stability: | Store dry |
Unit Definition: | One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO2 in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized. |
Hazard statements | H412 |
Precautionary statements | P273 - P501 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
activity | specific activity: ~0.4 units/mg protein (At 25 °C with formate as the substrate.) |
Storage Temp. | 2-8°C |
Enzyme Commission (EC) Number | 1.2.1.2 ![]() ![]() ![]() ![]() ![]() |
UNSPSC | 12352200 |