Formate Dehydrogenase
ROCHE/FORMD-RO - from Candida boidinii
Synonym: FDH; Formate:NAD oxidoreductase
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | yeast (Candida boidini) |
| color | off-white |
| concentration | ≥10-20 % (w/w) |
| foreign activity | ADH <0.0500% |
| LDH <0.0500% | |
| MDH <0.1000% | |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | 74 kDa |
| optimum pH | 7.5-8.5 |
| packaging | pkg of 250 U (10837016001) |
| pkg of 80 U (10244678001) | |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| solubility | water: soluble 10 g/L |
| specific activity | ~0.4 units/mg protein (At 25 °C with formate as the substrate.) |
| storage temp. | 2-8°C |
| suitability | suitable for gel electrophoresis |
| technique(s) | activity assay: suitable |
| UniProt accession no. | O13437  |
| Analysis Note: | Contaminants: <0.005% LDH and ADH each, <0.1% MDH |
| Application: | Most widely used in cofactor recycling systems for NADH. |
| Biochem/physiol Actions: | Substrate Specificity and Km: Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP. |
| General description: | Formate:NAD+ oxidoreductase Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO2 in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized. |
| Preparation Note: | Store dry |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~0.4 units/mg protein (At 25 °C with formate as the substrate.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.2.1.2 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |