Glutamate-Pyruvate Transaminase (GPT)
ROCHE/GPT-RO - from pig heart
Synonym: alanine aminotransferase
Product Type: Chemical
| biological source | pig heart |
| form | suspension |
| manufacturer/tradename | Roche |
| optimum pH | ~8.0 |
| packaging | pkg of 1 mL (10105589001 [10 mg]) |
| pkg of 2.5 mL (10737127001 [25 mg]) | |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ≥80 units/mg protein (At 25 °C with L-alanine and 2-oxoglutarate as the substrates.) |
| storage temp. | 2-8°C |
| Application: | Synthesis of unnatural L-amino acids from α-keto acids. |
| General description: | Glutamate-pyruvate transaminase is a blood enzyme. It metabolizes glutamate in blood resulting in low extracellular levels of brain glutamate. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Suspension in 3.2 M ammonium sulfate solution, pH approximately 6 |
| Preparation Note: | Activator: Pyridoxalphosphate Storage conditions (working solution): The enzyme is stable when dissolved in human serum and frozen in aliquots. For best results, centrifuge the enzyme and decant the supernatant before dissolving the enzyme in human serum to avoid high ammonium sulfate concentrations in the solution. |
| Quality: | Contaminants: < 0.01% GIDH, LDH, and MDH each, < 0.03% GOT |
| Unit Definition: | Unit Conversion: 1 U [+25 °C, pH 7.5] ≈ 1.75 U [+37 °C, pH 7.5] |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| activity | specific activity: ≥80 units/mg protein (At 25 °C with L-alanine and 2-oxoglutarate as the substrates.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.6.1.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |