Hexokinase/Glucose-6-Phosphate Dehydrogenase (HK/G6P-DH)
ROCHE/HKG6PDH-RO - from yeast/Leuconostoc, overproducer
Synonym: G6P-DH; Glucose-6-Phosphate DehydrogenaseHK; Hexokinase
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | yeast |
| color | white |
| concentration | 3.21 mg/mL |
| form | suspension |
| manufacturer/tradename | Roche |
| mol wt | dimer 110,000 Da |
| packaging | pkg of 10 mL (10737275001 [30 mg]) |
| pkg of 5 mL (10127825001 [15 mg]) | |
| pH | 5.5-6.5 |
| Quality Level | 100  |
| solubility | water: miscible |
| storage temp. | 2-8°C |
| suitability | suitable for UV spectrophotometry and general use |
| technique(s) | activity assay: suitable |
| Application: | Hexokinase/Glucose-6-phos |
| Components: | EC 2.7.1.1 & 1.1.1.49 |
| General description: | Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH. |
| Other Notes: | For life science research only. Not for use in diagnostic prodcedures. |
| Physical form: | Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content. |
| Unit Definition: | One unit (U) HK will phosphorylate 1 μmol of D-glucose in one minute at +25 °C and pH 7.6. One unit (U) G6P-DH will oxidize 1 μmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6. The coupled assay produces 1 μmol of NADH per μmol of D-glucose phosphorylated. Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |