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Hexokinase/Glucose-6-Phosphate Dehydrogenase (HK/G6P-DH)

ROCHE/HKG6PDH-RO - from yeast/Leuconostoc, overproducer

Synonym: G6P-DH; Glucose-6-Phosphate DehydrogenaseHK; Hexokinase

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-10127825001 5 mL
$236.00
1/EA
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45-10737275001 10 mL
$402.00
1/EA
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application(s) life science and biopharma
biological source yeast
color white
concentration 3.21 mg/mL
form suspension
manufacturer/tradename Roche
mol wt dimer 110,000 Da
packaging pkg of 10 mL (10737275001 [30 mg])
  pkg of 5 mL (10127825001 [15 mg])
pH 5.5-6.5
Quality Level 100 
solubility water: miscible
storage temp. 2-8°C
suitability suitable for UV spectrophotometry and general use
technique(s) activity assay: suitable
Application: Hexokinase/Glucose-6-phosphate dehydrogenase has been used for the determination of plasma glucose levels.
Components: EC 2.7.1.1 & 1.1.1.49
General description: Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH.
Other Notes: For life science research only. Not for use in diagnostic prodcedures.
Physical form: Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content.
Unit Definition: One unit (U) HK will phosphorylate 1 μmol of D-glucose in one minute at +25 °C and pH 7.6.
One unit (U) G6P-DH will oxidize 1 μmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 μmol of NADH per μmol of D-glucose phosphorylated.

Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) does not flash
Flash Point(C) does not flash
Storage Temp. 2-8°C
UNSPSC 12352200

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