Isopropyl-β-D-thiogalactoside

ROCHE/IPTG-RO - mol wt 238.3, pkg of 1 g (10724815001), pkg of 5 g (11411446001)

Synonym: Isopropyl β-D-1-thiogalactopyranoside; iptg; IPTG; Isopropyl β-D-thiogalactoside

CAS Number: 367-93-1
Empirical Formula (Hill Notation): C₉H₁₈O₅S
Molecular Weight: 238.30
MDL Number: MFCD00063273
Linear Formula: C₉H₁₈O₅S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-10724815001	1 g	$88.60 1/EA	Add To Favorites
45-11411446001	5 g	$324.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

form	powder
InChI	1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChI key	BPHPUYQFMNQIOC-NXRLNHOXSA-N
manufacturer/tradename	Roche
mol wt	238.3
packaging	pkg of 1 g (10724815001)
	pkg of 5 g (11411446001)
shipped in	wet ice
SMILES string	CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
storage temp.	2-8°C

Application:	IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Application:	Isopropyl-β-D-thiogalactoside (IPTG) is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. It has been used for the expression of recombinant genes in E. coli.
General description:	Isopropyl-β-D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Other Notes:	Non-metabolizable galactose analog.
Other Notes:	The amount of labeled DNA depends on: • amount of template DNA • purity of template DNA • conformation of template DNA • average size of labeled fragment
Preparation Note:	Sample Material 10 ng to 3μg template DNA, linearized and at least 100 to 200bp long
Preparation Note:	Working concentration: Final concentration should be 0.2 mM. Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.
Specifications:	Assay Time: 50minutes Specific Activity: The described standard assay will results in a specific activity of 1.8 x 10⁹dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352200

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