NAD

ROCHE/NAD100-RO - Grade I, free acid

Synonym: β-Nicotinamide adenine dinucleotide hydrate; β-DPN; β-NAD; Coenzyme 1; Cozymase; DPN; Diphosphopyridine nucleotide; NAD; Nadide

CAS Number: 53-84-9
Empirical Formula (Hill Notation): C₂₁H₂₇N₇O₁₄P₂ · xH₂O
Molecular Weight: 663.43 (anhydrous basis)
MDL Number: MFCD00036253
Linear Formula: C₂₁H₂₇N₇O₁₄P₂ · xH₂O
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-10127965001	1 g	$126.00 1/EA	Add To Favorites
45-10127973001	5 g	$467.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

λ_max	260 nm
assay	100% (dry weight)
color	off-white
form	lyophilized
InChI	1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1
InChI key	BAWFJGJZGIEFAR-NNYOXOHSSA-N
manufacturer/tradename	Roche
mol wt	M_r 663.4
packaging	pkg of 1 g (10127965001)
	pkg of 5 g (10127973001)
pH	~3.0 (50 mg/mL in water)
shipped in	wet ice
SMILES string	NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O
storage temp.	2-8°C

Application:	NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.
Application:	NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.
Biochem/physiol Actions:	Electron acceptor
Biochem/physiol Actions:	NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.
Biochem/physiol Actions:	The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.
General description:	Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD⁺ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Other Notes:	This is the common form of NAD.
Packaging:	Packaged by solid weight.
Preparation Note:	Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C
Quality:	Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A₂₅₀nm - A₂₆₀nm : 0.83 ± 0.02, A₂₈₀nm - A₂₆₀nm : 0.22 ± 0.02 Absence of contaminants: LDH inhibitors are not detectable Organic solvents: =0.05% (GC)
Specifications:	Absorbance coefficients: 17.6 x 10³l x mol^-1 x cm^-1 at 260nm, 5.9 x 10³l x mol^-1 x cm^-1 at 333nm (CN complex)

RIDADR	NONH for all modes of transport
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	100% (dry weight)
Storage Temp.	2-8°C
UNSPSC	12352200

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