N-Glycosidase F
ROCHE/NGLYF-RO - PNGase F of Flavobacterium meningosepticum, recombinant from E. coli
Synonym: glycosidase
Product Type: Chemical
| assay | ≥90% (SDS-PAGE) |
| conjugate | (N-linked) |
| form | solution |
| manufacturer/tradename | Roche |
| optimum pH | 7.0-8.0 |
| packaging | pkg of 0.1 mL (11365169001 [100 U]) |
| pkg of 0.25 mL (11365177001 [250 U]) | |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| specific activity | ~25000 units/mg protein |
| storage temp. | −20°C |
| Application: | Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide. Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol. |
| General description: | Peptide-N-glycosidase F, Peptide-N4-(acetyl-β-glucosaminyl)- |
| Legal Information: | The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycopeptide within 1 minute at 37 °C at pH 7.8. |
| Physical form: | Solution in 50 mM sodium phosphate, 12.5 mM EDTA, 50% glycerol (v/v), pH 7.2 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ~25000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.5.1.52 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |