Pefabloc® SC
ROCHE/PEFBSC-RO - powder, solubility: 100 mg/mL in aqueous buffer, suitable for blocking, suitable for protein purification
Synonym: Pefabloc® SC; 4-
Product Type: Chemical
Pefabloc® SC
Safety of Pefabloc® SC compared to PMSF and DFP. PMSF is a neurotoxin, and DFP is a deadly cholinesterase inhibitor. Mice were given oral doses of each inhibitor, and the LD50 in mg/kg was determined.
The effect of temperature and pH on the stability of Pefabloc® SC. Graph (A) shows the relative stability of Pefabloc® SC (5.0 mg/mL) in an aqueous phosphate buffer at +37 °C. Graph (B) shows the relative stability of Pefabloc® SC (5.0 mg/mL) in an aqueous phosphate buffer at pH 7.0.
| form | powder |
| manufacturer/tradename | Roche |
| mol wt | Mr 239.5 |
| mp | 175-185 °C |
| packaging | pkg of 1 g (11429876001) |
| pkg of 100 mg (11429868001) | |
| pkg of 500 mg (11585916001) | |
| Quality Level | 100  |
| solubility | aqueous buffer: 100 mg/mL |
| storage temp. | 2-8°C |
| technique(s) | blocking: suitable |
| protein purification: suitable |
| Application: | • Pefabloc SC is used to inhibit the detrimental effects of proteases during preparative protein purification. • Due to its low toxicity toward eukaryotic cells, it may be applied in the production of recombinant proteins, during fermentation of transformed cells, where proteolytic digestion may decrease the yield of the desired product. • Pefabloc SC is used to completely inactivate proteinase K during the preparation of chromosomal DNA in agarose plugs. For this purpose, the cells are embedded in agarose and digested with proteinase K to degrade all proteins. Before a specific restriction endonuclease is added, the proteinase K is inactivated by incubating the agarose plugs in 1 to 5 mM Pefabloc SC in 10 mM Tris-HCl, 1 mM EDTA , pH 7 for 2 hours at +37 °C or overnight. • In contrast to PMSF, Pefabloc SC is an excellent blocker of thrombin activity in serum or plasma. In these biological fluids, PMSF interacts in a reversible manner with albumin, which reduces its free concentration and leads to a delay in thrombin inactivation. Pefabloc SC, however, does not react with serum albumin, and exhibits a threefold higher capacity to inactivate thrombin under similar conditions. • Pefabloc SC can be used on living cells. • Use Pefabloc SC to inactivate proteinase K, for example, during pulsed-field gel electrophoresis (PFGE).With this technique, isolating the genomic DNA requires proteinase K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc SC inhibits proteinase K, and protects the stability of restriction enzymes used for further DNA analysis. |
| Features and Benefits: | • Benefit from an easy-to-use inhibitor. Add water-soluble Pefabloc SC directly to aqueous buffers. • Avoid hazardous compounds. Obtain non-toxic protease inhibition without risk to you, or those around you (see Figure 1). • Ensure protection with improved stability. Achieve consistent protease inhibition even at pH levels above 7.0 and temperatures above +4°C (see Figure 2). • Maximize inhibition. Be certain that your levels of active inhibitor are high enough by using a product that is reliably soluble and stable. |
| General description: | Pefabloc SC is a specific, potent, and irreversible inhibitor of serine proteases. The inhibitory activity is comparable to PMSF or DFP, but Pefabloc SC is non-toxic. |
| Legal Information: | Pefabloc is a registered trademark of Pentapharm |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: 0.1 to 1 mg/ml (0.4 to 4 mM) Working solution: Solvent is recommended in aqueous buffers or distilled water up to 100 mg/ml. Storage conditions (working solution): -15 to -25 °C (stock solution) The stability of Pefabloc SC in aqueous solution is affected both by pH and temperature. Concentrated stock solutions (e.g., 100 mM) prepared in dist. water are acidic and stable for 1 to 2 months at -15 to -25 °C, if stored in aliquots. Note: Store Pefabloc SC under acidic conditions and add to biological samples shortly before use to minimize hydrolysis. |
| Quality: | Function tested; homogeneous in TLC |
| Reconstitution: | Soluble up to 100 mg/ml in aqueous buffers or water. Stable in solution for 1 to 2 months if stored in aliquots at -15 to -25 °C. Only slight hydrolysis occurs under weakly basic conditions (pH 8.0 to 9.0). |
| Specificity: | Pefabloc SC belongs to the family of sulfonyl fluorides which irreversibly block serine proteases. Pefabloc SC was also described as potent serine threonine phosphatase inhibitor. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H314 |
| Precautionary statements | P260 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 |
| RIDADR | UN 3261 8 / PGII |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 175-185 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |