Pyruvate Kinase (PK)
ROCHE/PK-RO - from rabbit muscle
Synonym: PK
Product Type: Chemical
| biological source | rabbit muscle |
| form | suspension |
| manufacturer/tradename | Roche |
| optimum pH | 7.0-7.5 |
| packaging | pkg of 1 mL (10128155001 [10 mg]) |
| pkg of 10 mL (10128163001 [100 mg]) | |
| Quality Level | 100  |
| specific activity | ~200 units/mg protein (at 25 °C (500 U/mg at 37 °C) with PEP as the substrate.) |
| storage temp. | 2-8°C |
| Application: | Pyruvate kinase has been used to measure ATPase activity and in the determination of adenylate concentration. |
| Biochem/physiol Actions: | Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. Apart from this, the enzyme exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate. |
| General description: | ATP:pyruvate 2-O-phosphotransferase Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2+ and phosphoenolpyruvate. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Suspension in 3.2 M ammonium sulfate solution, pH approximately 6 |
| Preparation Note: | Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity. |
| Quality: | Contaminants: <0.001% GK, <0.002% “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| activity | specific activity: ~200 units/mg protein (at 25 °C (500 U/mg at 37 °C) with PEP as the substrate.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.7.1.40 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |