PMSF
ROCHE/PMSF-RO - Phenylmethylsulfonyl fluoride
Synonym: Phenylmethanesulfonyl fluoride; phenylmethylsulfonyl fluoride; α-Toluenesulfonyl fluoride; Benzylsulfonyl fluoride; PMSF; Phenylmethylsulfonyl fluoride
CAS Number: 329-98-6
Empirical Formula (Hill Notation): C7H7FO2S
Molecular Weight: 174.19
MDL Number: MFCD00007424
Linear Formula: C7H7FO2S
Product Type: Chemical
| assay | 98% (TLC) |
| concentration | 17-170 μg/mL |
| form | solid |
| InChI | 1S/C7H7FO2S/c8-11(9,10)6- |
| InChI key | YBYRMVIVWMBXKQ-UHFFFAOYSA |
| manufacturer/tradename | Roche |
| mol wt | 174.2 |
| mp | 92-94 °C |
| packaging | pkg of 10 g (10837091001) |
| pkg of 25 g (11359061001) | |
| pKa | 7.0 |
| Quality Level | 100  |
| shipped in | ambient |
| SMILES string | FS(=O)(=O)Cc1ccccc1 |
| solubility | >10 mg/mL (Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.) |
| storage temp. | 20-25°C |
| technique(s) | western blot: suitable |
| Application: | PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases. PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting. |
| Biochem/physiol Actions: | PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin. It is an irreversible inhibitor, and sulfonylates hydroxyl groups of the serine residues present in the active site of enzymes. |
| Other Notes: | Due to a short half-life, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may only have a half-life of 35 min. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: PK requires Mg2+ (or Mn2+, CO2+) and K+ (or NH4+, Rb+) for full activity. Working concentration: 17 to 170 μg/ml (0.1 to 1 mM) Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative. Storage conditions (working solution): 15 to 25 °C Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C. |
| Quality: | Purity: Chromatographically homogeneous |
| Reconstitution: | Soluble to > 10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at 15 to 25 °C. |
| Specificity: | Inhibits serine proteases such as chymotrypsin, trypsin, and thrombin, and the cysteine protease papain (reversible by DTT treatment). Does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases. |
| Symbol |   GHS05,GHS06 |
| Signal word | Danger |
| Hazard statements | H301 - H314 |
| Precautionary statements | P260 - P280 - P301 + P310 + P330 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 |
| RIDADR | UN 2923 6.1(8) / PGII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 98% (TLC) |
| mp | 92-94 °C |
| Storage Temp. | 20-25°C |
| UNSPSC | 12352200 |