Poly[d(I-C)]
ROCHE/POLYDIC-RO - lyophilized, pkg of 10 U (10108812001 [A<sub>260</sub> units]), pkg of 50 U (11219847001 [A<sub>260</sub> units])
Synonym: Poly-
Product Type: Chemical
| absorbance ratio | A250/260 nm 1.18-1.30 |
| A280/260 nm 0.61-0.67 | |
| A290/260 nm 0.24-0.32 | |
| color | white |
| description | Poly-deoxy-inosinic-deoxy |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | 28814 bp |
| mp | 54 °C (NaCl, 0.1 M, pH 7.0) |
| packaging | pkg of 10 U (10108812001 [A<sub>260</sub> units]) |
| pkg of 50 U (11219847001 [A<sub>260</sub> units]) | |
| Quality Level | 100  |
| solubility | water: soluble |
| storage temp. | −20°C |
| Analysis Note: | Typical analysis: Absorbance determination A250/A260, A280/A260, A290/A260 |
| Application: | Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations. It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA). |
| Features and Benefits: | Contents Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence |
| Other Notes: | 1 A260 unit corresponds to 50 μg ds DNA. Unit Conversion: 1 A260 unit corresponds to 50 μg ds DNA. |
| Other Notes: | Chain Length approx. 20,000 bp |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions. As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free. |
| Preparation Note: | Activator: Polymines (spermine, spermidine) Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 54 °C (NaCl, 0.1 M, pH 7.0) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |