Protein G Agarose
ROCHE/PROTGA-RO - >98% (HPLC and SDS-PAGE), suspension
Synonym: agarose; protein g
Product Type: Chemical
Protein G Agarose
| assay | >98% (HPLC and SDS-PAGE) |
| form | suspension |
| manufacturer/tradename | Roche |
| packaging | pkg of 15 mL (05015952001 [settled resin volume]) |
| pkg of 2 mL (11719416001 [settled resin volume]) | |
| pkg of 5 mL (11243233001 [settled resin volume]) | |
| storage temp. | 2-8°C |
| Analysis Note: | Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins. |
| Application: | Affinity chromatography using Protein G Agarose, like Protein A Agarose, is the method of choice for purification of IgG from many species. It has been used as a pre-clearing agent during- • Ubiquitination assay • Immunoprecipitation |
| Biochem/physiol Actions: | Protein G is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein G binds nearly all subclasses of IgG, but no other classes of immunoglobulins. Binding capacity: 20mg human IgG (polyclonal)/ml (the IgG is loaded at pH 7.0, and eluted with 200mM glycine, pH 2.8) Structure: recombinant Protein G (E. coli, Mr = 22,000) is covalently coupled to crosslinked 4% agarose beads: 2.5mg Protein G (>98% pure)/1ml gel. |
| Features and Benefits: | Contents Preswollen gel, ready-to-use, 2ml or 5ml gel (settled gel volume) in buffer that contains 14-19% ethanol as a preservative, nonsterile. Protein G content: 2.5mg/ml preswollen gel |
| General description: | Protein G was initially isolated from G148, a human group G Streptococcal strain. It is a cell wall protein and shows high affinity to IgG (immunoglobulin G). It has a putative molecular weight of 30,000Da. It interacts with all subclasses of human IgG and also binds to mouse, rabbit and goat IgG. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Symbol |   GHS02,GHS07 |
| Signal word | Warning |
| Hazard statements | H226 - H319 |
| Precautionary statements | P210 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378 - P403 + P235 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | 102.2 °F |
| Flash Point(C) | 39 °C |
| Purity | >98% (HPLC and SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 41116133 |