Anti-HA High Affinity
ROCHE/ROAHAHA - from rat IgG1
Synonym: antibody
Product Type: Chemical
Anti-HA High Affinity
Immunoprecipitation of an HA-tagged protein with Anti-HA High Affinity. HA-tagged green fluorescent protein (GFP) was immunoprecipitated by incubation with the indicated amounts (in μg) of Anti-HA High Affinity, followed by incubation with Protein-G-agarose. Pellets were solubilized, electrophoresed, and western blotted. Detection was achieved using Anti-HA-Peroxidase and the BM Chemiluminescence Western Blotting Substrate. Note that approximately 20-fold less antibody is required for efficient immunoprecipitation using antibody from the high affinity 3F10 clone
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 3F10, monoclonal |
| conjugate | unconjugated |
| epitope sequence | YPYDVPDYA |
| form | lyophilized |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 μg (11867423001) |
| pkg of 500 μg (11867431001) | |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Use Anti-HA High Affinity for the detection of native influenza hemagglutinin protein and recombinant proteins that contain the HA epitope using: • Dot blots • ELISA • Immunocytochemistry • Immunoprecipitation • Western blots Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling. |
| General description: | Anti-HA High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10). Anti-HA High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging". |
| Immunogen: | Amino acids 98-106 from the human influenza virus hemagglutinin protein |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • ELISA: for detection 100 ng/ml; for coating 1 to 5 μg/ml • Immunoprecipitation: 0.5 to 5 μg/ml • Western blot: 50 to 200 ng/ml |
| Quality: | Function tested in western blot. |
| Reconstitution: | Add 0.5 ml (11 867 423 001; 50 μg) or 2.5 ml (11 867 431 001; 500 μg) double-distilled water to a final concentration of 100 μg/ml or 200 μg/ml respectively. Rehydrate for 10 min prior to use. |
| Specificity: | Anti-HA, High Affinity recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal). |
| Symbol |  GHS07,GHS07 |
| Signal word | Warning-Warning |
| Hazard statements | H317 - H412 - H317 |
| Precautionary statements | P261 - P273 - P280 - P261 - P272 - P280 - P333 + P313 - P362 + P364 - P333 + P313 - P362 + P364 - P501 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |