Amyloglucosidase
ROCHE/ROAMYGLL - from Aspergillus niger
Synonym: Aspergillus niger; disaccharidase-type a-glucosidase
Product Type: Chemical
| biological source | Aspergillus niger |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | 97 kDa |
| optimum pH | 4.6-4.8 |
| packaging | pkg of 3,500 U (11202367001) |
| pkg of 500 U (11202332001) | |
| Quality Level | 100  |
| specific activity | 6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate) |
| ~6 units/mg protein (At 25 °C with glycogen as the substrate.) | |
| storage temp. | 2-8°C |
| Application: | Amyloglucosidase from Aspergillus niger is used for the determination of starch. |
| Biochem/physiol Actions: | Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup. |
| Biochem/physiol Actions: | Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose. |
| General description: | Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • Western blot: 1 to 10 μg/ml Working solution: Tris-buffered saline containing 0.1% Tween 20. Storage conditions (working solution): 2 to 8 °C |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 - P342 + P311 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: 6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate); specific activity: ~6 units/mg protein (At 25 °C with glycogen as the substrate.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |