Trypsin recombinant, Proteomics Grade
ROCHE/RTRYP-RO - recombinant enzyme expressed in Pichia pastoris
Synonym: Trypsin
Product Type: Chemical
| assay | 95% (gel filtration) |
| form | lyophilized |
| manufacturer/tradename | Roche |
| packaging | pkg of 4 × 100 μg (03708969001) |
| pkg of 4 × 25 μg (03708985001) | |
| Quality Level | 100  |
| recombinant | expressed in Pichia pastoris |
| specific activity | ≥150 units/mg protein |
| storage temp. | −20°C |
| Application: | Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry. |
| General description: | This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Stabilizers: Ca2+ in mM concentration range Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain. Storage conditions (working solution): -15 to -25°C The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing! |
| Reconstitution: | Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing. |
| Specificity: | Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | 95% (gel filtration) |
| activity | specific activity: ≥150 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |