Taq DNA Polymerase, 5 U/μl
ROCHE/TAQ-RO - optimum pH ~9.0 (20 °C), optimum reaction temp. 72 °C
Synonym: dna amplification; pcr; polymerase, pcr, primer extension, dna amplification; primer extension
Product Type: Chemical
Taq DNA Polymerase, 5 U/μl
Roche Taq DNA Polymerase has high lot-to-lot consistency. Five different lots of Taq DNA Polymerase (lanes 1-5) were tested using a 0.5 kb fragment of λDNA. PCR products were visualized by gel electrophoresis. Result: Consistent amplifications were obtained
Taq DNA polymerase
| application(s) | genomic analysis life science and biopharma |
| biological source | bacterial (Thermus aquaticus) |
| color | colorless |
| foreign activity | Nicking activity 30 units, none detected |
| Ribonuclease 30 units, none detected | |
| Unspecific endonucleases 30 units, none detected | |
| form | liquid |
| grade | Molecular Biology |
| manufacturer/tradename | Roche |
| mol wt | 95 kDa |
| NCBI accession no. | AAA27507   |
| optimum pH | ~9.0 (20 °C) |
| packaging | pkg of 1,000 U (11418432001 [4 x 250 U]) |
| pkg of 100 U (11146165001) | |
| pkg of 2,500 U (11596594001 [10 x 250 U]) | |
| pkg of 5,000 U (11435094001 [20 x 250 U]) | |
| pkg of 500 U (11146173001 [1 x 500 U]) | |
| parameter | 72 °C optimum reaction temp. |
| Quality Level | 100 |
| recombinant | expressed in E. coli |
| solubility | water: miscible |
| specific activity | 5 U/μL |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | DNA sequencing: suitable |
| PCR: suitable | |
| UniProt accession no. | P19821 |
| usage | sufficient for ≤1,000 reactions (11146173001) |
| sufficient for ≤10,000 reactions (11435094001) | |
| sufficient for ≤2,000 reactions (11418432001) | |
| sufficient for ≤200 reactions (11146165001) | |
| sufficient for ≤5,000 reactions (11596594001) |
| Application: | Taq DNA Polymerase can be used in simple, routine PCR. Roche Applied Science Taq DNA polymerase is held to rigorous purity and quality standards. This preparation of recombinant Taq DNA Polymerase can be applied for: • PCR • RT-PCR • qPCR • Other primer-extension reactions, such as sequencing and labeling |
| Features and Benefits: | Reliable reproducible results: High lot-to-lot consistency. No need to test each lot: Taq DNA Polymerase is rigorously tested. Prevent PCR carryover: dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination. |
| General description: | Taq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa. Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme was cloned in E.coli. |
| Legal Information: | Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 2 components |
| Quality: | Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9. Lack of restriction endonuclease: The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures. |
| Unit Definition: | One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetripho Unit Assay: Incubation buffer: 67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP. Incubation procedure: M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation. Volume Activity: 5 U/μl |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: 5 U/μL |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.7 ( BRENDA | IUBMB ) |
| UNSPSC | 41106300 |
| Components | Taq DNA Polymerase 5 U/μl; PCR Buffer with MgCl<sub>2</sub> 10x concentrated; MgCl<sub>2</sub> Stock Solution; PCR Buffer without MgCl<sub>2</sub> |