Trypsin Sequencing Grade
ROCHE/TRYPSEQ-RO - from bovine pancreas
Synonym: trypsin
Product Type: Chemical
| biological source | bovine pancreas |
| form | lyophilized (salt-free) |
| manufacturer/tradename | Roche |
| mol wt | Mr 23.5 kDa |
| optimum pH | 8.0 |
| packaging | pkg of 4 × 100 μg (11047841001) |
| pkg of 4 × 25 μg (11418475001) | |
| Quality Level | 100  |
| specific activity | ≥80 units/mg protein |
| storage temp. | 2-8°C |
| Application: | Trypsin Sequencing Grade is used to to digest proteins in solution, in gels or on blotting membranes. The enzyme is used for protein-structure elucidation, tryptic mapping, fingerprinting, sequence analysis, and translocation studies. Trypsin Sequencing Grade generates glycopeptides from purified glycoproteins and is suited for the digestion of proteins in polyacrylamide gels. |
| General description: | Trypsin Sequencing Grade is isolated from bovine pancreas as a highly purified and specific protease. Trypsin Sequencing Grade is a serine endopeptidase. It specifically cleaves peptide bonds at the carboxylic side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Stabilizers: Trypsin is stable in 4 M. Working concentration: 1/100 to 1/20 of the protein by weight (in solution); 1-5 μg/100 μl (for in-gel digest) Storage conditions (working solution): A solution in 0.01% trifluoroacetic acid (TFA), (v/v) or 1 mM HCl may be used for one week at maximum, if stored at 2 to 8 °C. By incubation of proteins in solution at neutral to slightly basic pH-values partial autolysis might occur. For this application, Roche recommends Trypsin, Modified, Sequencing Grade. |
| Quality: | Purity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC. |
| Reconstitution: | Reconstitution in acid is necessary for stability of solution: 0.01% TFA (v/v), 1 mM HCl or 0.1% acetic acid are recommended. |
| Specificity: | The specificity of Trypsin Sequencing Grade is verified with the oxidized B-chain of insulin (insulin Box) as substrate. High concentrations of Trypsin Sequencing Grade (1 part by weight enzyme with 18 parts by weight insulin Box) are incubated for 18 hours to detect traces of chymotrypsin impurities. Specificity (HPLC, with Insulin Box): Cleavage after 1 hour: ≥90%; Unspecific cleavage products after 18 hours: ≤10% |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ≥80 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |